Section 1.0 - Laboratory Safety and Maintenance

Section 2.0: Tissue

Section 3.0: General Cell Culture SOPs

Section 4.0: Media and Aliquot Preparation

Section 5.0: Staining Procedures

Section 6.0: Animal Procedures and Information

Section 7.0: Molecular Biology SOPs

Section 8.0: FACS Procedures

8.1 Aldefluor Enzymatic Assay

8.2 FACS for Aldefluor in MCF-10

8.3 FACS for ALDH1 in MCF-10A Cells

8.4 FACS for CD29-PE and Aldefluor

8.5 FACS for c-Met and Aldefluor

8.6 FACS for c-Met and H2Kd

8.7 FACS Staining

8.8 Flow Protocol

8.9 Flow Cytometry Preparation

8.10 Procedure for Making Baaa Aliquots for Aldefluor

8.11 FACS for Aldefluor

8.12 CD24/CD44 Flow Cytometry Protocol

8.13 FACS for H2Kd/APC-Cy7 for Tumor Cells from Mice

8.14 FAC Staining of Human Breast Cancer Xenografts for ALDH, CD24, CD44, EpCAM and Mouse Cells

8.15 FACS Staining of Human Breast Cell Lines for ALDH, CD24, CD44, EpCAM, CD90 and CD49f

8.16 FACS Staining of Human Breast Cell Lines for ALDH, CD24, CD44, EpCAM, and DAPI

8.17 FACS Staining of Human Breast Cancer Cores for ALDH, CD24, CD44, EpCAM and Lineage Negative Cells

Section 9.0: Additional Wicha Lab Protocols

Section 10.0: Additional SOPs and Reference Material