Section 1.0 - Laboratory Safety and Maintenance

Section 2.0: Tissue

Section 3.0: General Cell Culture SOPs

Section 4.0: Media and Aliquot Preparation

Section 5.0: Staining Procedures

Section 6.0: Animal Procedures and Information

Section 7.0: Molecular Biology SOPs

Section 8.0: FACS Procedures

8.1 Aldefluor Enzymatic Assay

8.2 FACS for Aldefluor in MCF-10

8.3 FACS for ALDH1 in MCF-10A Cells

8.4 FACS for CD29-PE and Aldefluor

8.5 FACS for c-Met and Aldefluor

8.6 FACS for c-Met and H2Kd

8.7 FACS Staining

8.8 Flow Protocol

8.9 Flow Cytometry Preparation

8.10 Procedure for Making Baaa Aliquots for Aldefluor

8.11 FACS for Aldefluor

8.12 CD24/CD44 Flow Cytometry Protocol

8.13 FACS for H2Kd/APC-Cy7 for Tumor Cells from Mice

Section 9.0: Additional Wicha Lab Protocols

Section 10.0: Additional SOPs and Reference Material