Section 1.0 - Laboratory Safety and Maintenance

Section 2.0: Tissue

Section 3.0: General Cell Culture SOPs

3.1 Cell Counting

3.2 Coulter Counting

3.3 Cell Freeze and Thaw

3.4 Cell Culture Media

3.5 Cell Passage Guidelines

3.6 Trypan Blue Preparation

3.7 RBC Lysis

Section 4.0: Media and Aliquot Preparation

Section 5.0: Staining Procedures

Section 6.0: Animal Procedures and Information

Section 7.0: Molecular Biology SOPs

Section 8.0: FACS Procedures

Section 9.0: Additional Wicha Lab Protocols

Section 10.0: Additional SOPs and Reference Material