Section 1.0 - Laboratory Safety and Maintenance

Section 2.0: Tissue

Section 3.0: General Cell Culture SOPs

Section 4.0: Media and Aliquot Preparation

Section 5.0: Staining Procedures

Section 6.0: Animal Procedures and Information

6.1 Mouse Ear Tagging

6.2 Mouse Room Door Combinations

6.3 Ketamine/Xylazine Containing Anesthesia for Mouse Surgery Prep

6.4 Directions for Constructing a Mouse Tail Vein Injection Apparatus

6.5 Peripheral Blood Collection Protocol

6.6 Murine Xenotransplantation of Primary Tumor Cells, Tumorigenic Cell Lines or Normal Mammary Epithelial Cells

6.7a Tumor Implantation into Mice

6.8 NCRC Animal Imaging Procedures

6.9 BSRB Agents in Mice in BSRB Imaging Center

6.10 Guidelines for the Use of Taxotere in Mice

6.11 Mouse Anesthesia via Isoflurane Jar

6.12 Mouse Anesthesia via Isoflurane Nebulizer

6.13 Mouse Euthanasia

6.14 Intra-medullary Tibia Injection

6.15 Estradiol Pellet Implantation

6.16 Ordering Controlled Substances for Research Use

Section 7.0: Molecular Biology SOPs

Section 8.0: FACS Procedures

Section 9.0: Additional Wicha Lab Protocols

Section 10.0: Additional SOPs and Reference Material