Section 1.0 - Laboratory Safety and Maintenance

Section 2.0: Tissue

Section 3.0: General Cell Culture SOPs

Section 4.0: Media and Aliquot Preparation

4.1 Complete Media Preparation

4.2 Agarose Plate Preparation

4.3 Preparation of Sodium Selenite

4.4 Preparation of BSA

4.5 Preparation of HC

4.6 Preparation of Insulin

4.7 Preparation of Medium for ER Expression

4.8 Preparation of T3

4.9 Preparation of Transferrin

4.10 Preparation of Ampicillin

4.11 Preparation of bFGF

4.12 Preparation of Collegenase

4.13 Preparation of EGF

4.14 Preparation of Fungizone

4.15 Preparation of HEPES

4.16 Preparation of PBS

4.17 Preparation of Cholera Toxin

4.18 Preparation of Serum + EGF Medium

4.19 Preparation of Serum Free Medium

4.20 Preparation of ELISA Reagents/Buffers

4.21 Preparation of Dispase Aliquots

4.22 Preparation of Collagenase for Tumor Digestion

4.23 Preparation of DNase

4.24 Preparation of DAPI

4.25 Preparation of methyl cellulose 10x for mammosphere assay

4.26 Preparation of L-Glutamine

4.27 Preparing Luciferin

Section 5.0: Staining Procedures

Section 6.0: Animal Procedures and Information

Section 7.0: Molecular Biology SOPs

Section 8.0: FACS Procedures

Section 9.0: Additional Wicha Lab Protocols

Section 10.0: Additional SOPs and Reference Material