Section 1.0 - Laboratory Safety and Maintenance

Section 2.0: Tissue

2.1 Tissue Collection

2.2 Human Tumor Digestion

2.3 Dissociation of Mammary Tissue

2.4 Dissociation of Cells [Mouse Xenograft Tumor]

2.5 Dissociation of Mammospheres

2.5a Dissociation of Spheres

2.6 Organoids

2.7 Single Cell Suspension from Primary Tumors

2.8 Mammosphere Culture and Dissociation

2.9 Expanding Frozen Xenograft Stock

2.10 Dissociation of Primary Tumor to get Cancer Associated Fibroblasts (CAFs)

Section 3.0: General Cell Culture SOPs

Section 4.0: Media and Aliquot Preparation

Section 5.0: Staining Procedures

Section 6.0: Animal Procedures and Information

Section 7.0: Molecular Biology SOPs

Section 8.0: FACS Procedures

Section 9.0: Additional Wicha Lab Protocols

Section 10.0: Additional SOPs and Reference Material