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Service Estimates

Estimates for Frequently Used Hybridoma Core Services

Monoclonal Antibody Development Project
The investigator’s laboratory is responsible for screening of sera and culture supernatants. Consultation is available to help develop methods.  Costs will increase if more clones are expanded, cryopreserved, subcloned and etc.

Service

Rate

Total

Immunize, 3 BALB/c mice (1 i)

$1002

$1002

Fusion, 1 spleen, 480 X 96 wells (1 f2)

$1170

$1170

Expand 20 primary cultures for rescreening (20 tc1)

$34

$680

Expand 10 primary cultures, cryopreserve two vials each (10 tc2, 10 cryo)

$76

$760

Subclone 4 primary cultures (4 c)

$150

$600

Expand  2 clones from each of  4 primary cultures to 24 well plate culture (8 tc1)

$34

$272

Expand 8 sub-clones and cryopreserve two vials each (8 tc2, 8 cryo)

$72

$608

Grow cells from two hybridomas for ascites productions (4 tc3)

$32

$128

Antibody production, in vivo, 2 Balb/c mice per cell line, 2 hybridomas (2 ap1)

$450

$900

Isotype determination, 2 hybridomas (2 iso)

$46

$92

Estimated Total Cost

 

$6212

 

Thaw and Cryopreserve a Hybridoma Cell Line
Approximately 50 ml of culture supernatant can be saved from this work and provided to lab if requested in advance.

Service

Rate

Total

Thaw 1 vial cells (1 ct)

$40

$40

Expand culture (1 tc2)

$38

$38

Grow cells for cryopreservation (5 tc3)

$32

$160

Freeze cells (3 cryo = 6 vials, includes storage in our facility for 5 years)

$38

$114

Estimated Total Cost

 

$352

 

Monoclonal Antibody Production in vitro in CELLine CL-1000 high density culture
Estimate does not include thawing or cryopreserving cells or weaning cells to a low serum growth media.  Harvest antibody concentrations typically range from 0.25 – 2.5 mg/ml; the total harvest volume in 30 days ranges from 60 - 120 ml.  CL-1000 flasks may be maintained and harvested for a longer time at an additional cost of  $1164.00 for each 30 day period.

Service

Rate

Total

Grow cells to innoculate flask (6 tc4)

$25

$150

Set up CL-1000 flask, culture 30-45 days, collect 4-6 harvests @ approx. 15 ml ea (1 ap5)

$1445

$1445

Estimated Total Cost

 

$1595

Monoclonal Antibody Production as Ascites in 2 BALB/c Mice
Estimate does not include thawing or cryopreserving cells.  Antibody concentration typically ranges from 1 - 10 mg/ml and volume yields range from 5 – 20 ml.

Service

Rate

Total

Grow cells to inject mice (2 tc3)

$32

$64

in vivo antibody production, 1 hybridoma, 2 Balb/c mice (1 ap1)

$450

$450

Estimated Total Cost

 

$514