Skip Navigation

Recharge Fees

Hybridoma Core services are available to all University of Michigan investigators. 

The Hybridoma Facility accepts requests from off-campus users on a contract basis, subject to on-campus demand.  Please contact (link to contact info) the Core to request an estimate of the cost for these services.

Hybridoma Core services are charged in an “a la carte” fashion so that the investigator is charged only for the work that is done.  For example, if the mice do not respond to your antigen, you pay only for the immunization.  When a fusion is screened, you pay individually to expand and work with each clone you select.  Some examples of estimated costs for typical uses of the Hybridoma Core are found here (make here a hyperlink to separate page service estimates).

All rates are subject to change as determined by the cost to provide the service(s) under the policies of the University of Michigan Office of Financial Analysis.





Immunization (in vivo, 3 mice)






Spleen harvest with cryopreservation of dissociated cells, 1 spleen

tc 1

Tissue Culture 1 (culture one cell line from one 96-well to one 24-well)


tc 2

Tissue Culture 2 (culture one cell line from one 24-well to two 6-wells)


tc 3

Tissue Culture 3 (culture one cell line, each 6-well (two through six)


tc 4

Tissue Culture 4 (cultureone cell line, each 6-well (seven through twelve)


clm 1

Cell line maintenance 1 (maintain one cell line in one 96-well for one week)


clm 2

Cell line maintenance 2 (maintaining one cell line in one 24-well for one week)


clm 3

Cell line maintenance 3 (maintaining one cell line in one 6-well for one week)



cloning one cell line through screening of subclones



Cryopreservation (per cell line, two cryovials)



Cell Thawing (per cell line)


ap 1

In vivo antibody production (ascites, two mice)


ap 2

In vivo antibody production (ascites, two immunosuppressed mice)


ap 3

In vivo antibody production (ascites, two athymic nude mice)


ap 4

In vivo antibody production (ascites, five athymic nude mice)


ap 5

In vitro antibody production (CL1000 flask set up with harvests for over 30 day period)



Isotyping (per antibody, Iso-strip or ELISA method )



Cell line storage (per vial, per year)