Plasmid Midi Prep (~100 μg)

Custom purified DNA prep, where the yield is approximately 100 μg.

$60

$100

Plasmid Mega Prep (~1000 μg)

Custom purified DNA prep, where the yield is approximately 1000 μg.

$365

$605

Plasmid Transformation

This process is used for the batch transformation of plasmid DNA into E. Coli cells, for plasmid amplification.

$60

$100

Stock Plasmid DNA, Ready-made (5 μg)

Each sample contains 5 μg of DNA.

$45

Inquire**

Name

Map

Sequence

Description

pACCMV2

Adenoviral shuttle plasmid with CMV promoter, pUC19 polylinker, SV40 splice/polyA, loxP and unique restriction tag SwaI, PmeI, NheI for linearization. Updated version of pACCMVpLpA(-)loxp-SSP. Shuttle used to produce Ad5 E1/E3-deleted virus.

pACRSV2

Adenoviral shuttle plasmid with RSV promoter, pUC19 polylinker, SV40 splice/polyA, loxP and unique restriction tag SwaI, PmeI, NheI for linearization. Updated version of pARSVpLpA(-)loxp-SSP. Shuttle used to produce Ad5 E1/E3-deleted virus.

pAdEF1 alpha loxP

Adenoviral shuttle plasmid with EF1 alpha promoter, polylinker, bovine growth hormone polyA site, loxP and unique restriction site NheI for linearization. Shuttle used to produce Ad5 E1/E3-deleted virus.

pACpL+loxP-SSP

Adenoviral shuttle plasmid with pUC19 polylinker and unique restriction tag SwaI, PmeI, NheI for linearization. Does not contain promoter or polyA site. Shuttle used to produce Ad5 E1/E3-deleted virus.

pACCMVpLpA(-) loxP-SSP

Adenoviral shuttle plasmid with CMV promoter, pUC19 polylinker, SV40 splice/polyA, loxP and unique restriction tag SwaI, PmeI, NheI for linearization. Shuttle used to produce Ad5 E1/E3-deleted virus.

pACRSVpLpA(-) loxP-SSP

Adenoviral shuttle plasmid with RSV promoter, pUC19 polylinker, SV40 splice/polyA, loxP and unique restriction tag SwaI, PmeI, NheI for linearization. Shuttle used to produce Ad5 E1/E3-deleted virus.

pACCMVpLmP1 (-) loxP-SSP

Adenoviral shuttle plasmid with CMV promoter, pUC19 polylinker, mP1 splicing signal/poly A, loxP with unique restriction tag SwaI, SfiI, PmeI for linearization. Shuttle used to produce Ad5 E1/E3-deleted virus.

Name

Map

Sequence

Description

pRET10

N/A

N/A

Proviral plasmid used in the construction of recombinant MoMLV. This plasmid contains a bicistronic element to express both a transgene and GFP marker driven by one CMV promoter through the use of an IRES element. The CMV promoter is followed by an intron region flanked with donor and acceptor splicing sites before the multi-splicing site.

pRET6

N/A

N/A

Proviral plasmid used in the construction of recombinant MoMLV. This plasmid contains a bicistronic element to express both a transgene and puromycin selectable marker driven by one CMV promoter through the use of an IRES element. The CMV promoter is followed by an intron region flanked with donor and acceptor splicing sites before the multi-splicing site.

Name

Map

Sequence

Species

Description

pLentilox 3.7 GFP

rHIV

Proviral plasmid used in shRNA construction in which shRNA expression is driven through a U6 promoter. GFP marker contained in the vector is expressed through a separate CMV promoter downstream of the U6-siRNA expression cassette. This allows simultaneous expression of both shRNA and fluorescent marker. GFP is co-integrated into host cell genome with shRNA which allows for convenient tracking of viral uptake in the cell and selection through fluorescent visualization.

pLentilox 3.7 dsRed

rHIV

Proviral plasmid used in shRNA construction in which shRNA expression is driven through a U6 promoter. The dsRed marker contained in the vector is expressed through a separate CMV promoter downstream of the U6-siRNA expression cassette. This allows simultaneous expression of both shRNA and selectable marker. dsRed is co-integrated into host cell genome with shRNA which allows for convenient tracking of viral uptake in the cell and selection through fluorescent visualization.

pLentilox 3.7 Puro

rHIV

Proviral plasmid used in shRNA construction containing a Puromycin selectable marker. U6 promoter is upstream of CMV-Puromycin expression cassette for simultaneous production of shRNA and selectable marker. Puromycin co-integrates into host cell genome which allows for convenient, stable selection.

pLentilox RSV GFP

rHIV

Transgene expression of this proviral plasmid is driven by an RSV promoter. Vector also contains separate expression cassette for GFP marker utilizing a CMV promoter. GFP is co-integrated into host cell genome and allows for convenient tracking of viral uptake in the cell and selection through fluorescent visualization.

pLentilox RSV2dsRed

rHIV

Transgene expression of this proviral plasmid is driven by an RSV promoter. Vector also contains separate expression cassette for dsRed marker utilizing a CMV promoter. dsRed is co-integrated into host cell genome and allows for convenient tracking of viral uptake in the cell and selection through fluorescent visualization.

pLentilox RSV puro

rHIV

Transgene expression of this proviral plasmid is driven by an RSV promoter. Vector also contains separate expression cassette for puro marker utilizing a CMV promoter. Puro is co-integrated into host cell genome and allows for convenient tracking of viral uptake in the cell and puromycin selection.

pLentilox IRES GFP

rHIV

Pro-viral plasmid containing a bicistronic element to express both a transgene and GFP marker driven by one CMV promoter through the use of an IRES element. IRES elements tend not to be as strong as typical promoter expression cassettes and therefore could result in a weaker GFP signal.

pLentilox IRES Puro

rHIV

Proviral plasmid containing a bicistronic element to express both a transgene and a puromycin selectable marker driven by one CMV promoter through the use of an IRES element. IRES elements tend not to be as strong as typical promoter expression cassettes and therefore could result in a weaker puromycin response.

pLentilox EV (empty)

rHIV

Pro-viral plasmid in which trans-gene expression is driven by a CMV promoter. Vector contains no selectable marker. The use of the CMV promoter is ideal for transgene over-expression in a wide range of cell types.

pLenti6/V5-DEST

N/A

N/A

rHIV

This Invitrogen expression system utilizes the Gateway Technology of gene transfer. This vector is ideal for multiple expression systems. Contains blasticidin resistance gene for stable selection. This plasmid is fully compatible with Vector Core system for custom lentivirus production.

pLenti6/V5-GW/LacZ

N/A

N/A

rHIV

This Invitrogen expression system utilizes the Gateway Technology of gene transfer. This vector allows simple sub-cloning of trans-gene. Blasticidin resistance gene for stable selection. This plasmid is fully compatible with Vector Core system for custom lentivirus production.

pLentilox EV-GFP

rHIV

Proviral plasmid in which transgene expression is driven by a CMV promoter. Vector contains the GFP marker. The use of the CMV promoter is ideal for transgene over-expression in a wide range of cell types.

pLentilox EV-Luc

rHIV

Proviral plasmid in which transgene expression is driven by a CMV promoter. Vector contains the luciferase (firefly) marker. The use of the CMV promoter is ideal for transgene over-expression in a wide range of cell types.

Lenti-FIV-GFP

N/A

N/A

rFIV

Recombinant Feline Immunodeficiency Virus (FIV) reporter vector expressing the GFP marker. Expression is driven from a RSV promoter.

Lenti-FIV-LacZ

N/A

N/A

rFIV

Recombinant Feline Immunodeficiency Virus (FIV) reporter vector expressing the β-gal marker. Expression is driven from an RSV promoter.

Name

Map

Sequence

Description

pUMVC1-CAT

Mammalian expression vector for the expression of chloramphenicol resistance. CMV IE Promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal - chloramphenicol transferase.

pUMVC1-hGM-CSF

CMV IE Promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal - human granulocyte-monocyte colony stimulating factor.

pUMVC1-hpAP

CMV IE Promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal - human placental alkaline phosphatase.

pUMVC1-mGM-CSF

CMV IE Promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal - mouse granulocyte-monocyte colony stimulating factor.

pUMVC1-nt-beta-gal

CMV IE Promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal - nuclear targeted beta-galactosidase.

pUMVC1-tk

CMV IE Promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal - mammalian expression vector for HSV1 thymidine kinase.

pUMVC3-4070a-env

Expresses Moloney mouse leukemia virus gPr80 envelope polyprotein precursor. This precursor is proteolytically cleaved into gp70SU and p15TM subunits. Amphotropic 4070A virus envelope.

pUMVC3-gag-pol

Plasmid expresses gag-pol genes from Moloney murine leukemia virus for co-tranfection with expression vector and appropriate env expressing plasmid. Retrovirus gag-pol helper plasmid, kanamycin resistant.

pUMVC3-hFL

Mammalian expression vector for human Flt3 ligand, full length.

pUMVC3-hFLex

Mammalian expression vector for human Flt3 ligand, secreted.

pUMVC3-hIFN alpha 2

CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal, expresses human interferon alpha 2.

pUMVC3-hIL10

Mammalian expression vector for human interleukin 10.

pUMVC3-hIL12

CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal, internal ribosome entry site, p35, p40 - human interleukin-12.

pUMVC3-hIL15

CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal, kanamycin resistance - mammalian expression vector for human interleukin-15.

pUMVC3-hIL2

CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal - mammalian expression vector for human interleukin-2.

pUMVC3-hIL2/IL15

Human interleukin 2 signal peptide fused to human interleukin15 mature peptide. CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal, kanamycin resistance, human interleukin-2/interleukin-15 fusion.

pUMVC3-hIL4

A mammalian expression vector for human interleukin 4.

pUMVC3-hIL7

Mammalian expression vector for human interleukin 7.

pUMVC3-mFL

Mammalian expression vector for mouse FLT3 ligand.

pUMVC3-mFLex

Mammalian expression vector expressing a secreted form of mouse FLT3 ligand.

pUMVC3-mIL10

Mammalian expression vector for mouse interleukin 10, CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal

pUMVC3-mIL12

CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal, internal ribosome entry site, p35, p40 - mammalian expression vector for mouse interleukin 12.

pUMVC3-mIL2

CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal - mammalian expression vector for mouse interleukin 2.

pUMVC3-mIL4

Mammalian expression vector for mouse interleukin 4.

pUMVC3-mIL7

Mammalian expression vector for mouse interleukin 7.

pUMVC3-shIL15R

Expression vector for the extracellular domain of human interleukin 15 receptor alpha -soluble human interleukin 15 receptor alpha.

Name

Map

Sequence

Description

pUMVC1

CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal - PstI, SalI, EcoRV, BglII polylinker, kanamycin resistance.

pUMVC2

CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal - Expression plasmid, CMV promoter, polylinker deleted, rabbit beta-globin poly A, kanamycin resistance.

pUMVC3

Mammalian expression plasmid CMV IE promoter/ enhancer/trimmed Intron A, rabbit beta-globin polyadenylation signal -  kanamycin resistance.

pUMVC4a

CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal, kanamycin resistance, ISS from ampicillin resistance gene (+) orientation - immunostimulatory sequence.

pUMVC4b

CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal, kanamycin resistance, ISS from ampicillin resistance gene (-) orientation - immunostimulatory sequence.

pUMVC6

CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal, kanamycin resistance, modified interleukin-2 secretion signal sequence - secretion signal peptide, immunostimulatory sequence.

pUMVC7

Can be used to retarget intracellular peptides to a secretory pathway by fusion to the tissue plasminogen activator signal peptide. - pUMVC7, CMV promoter, tPA signal peptide.