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Quick Facts
Vector Core
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| Product-plasmids and stock virus | Service | Rate* (Internal) | Rate (External) | Turn-Around Time |
Notes |
| Adenovirus | Adenoviral - Construction with Plaque Amplification | $595 | $2,985 (includes: construction, large scale purification and titer) | 4 weeks for construction (minimum). For external users: Additional time needed for amplification (2 weeks) and titering (2 weeks). | For each construction, 50 μg of shuttle DNA (purified and completely cut by either PmeI or NheI) is required. For custom construction of commercial vector systems that are already full length (such as AdenoX, Adeasy), please linearize the plasmid according to the system specifications. |
| Adenoviral - Large Scale Purification and Expansion | $1,050 | $2,005 (includes titer) | 2 weeks | Need 100 μl of lysate or 10 μl purified. Make sure the virus has not been serially propagated. | |
| Adenoviral - Standard Glycerol Stock | $130 | $215 | same day | Pre-made stocks of popular viral vectors at 1012 particles. Titer is included. Each sample is 0.25 mls. Store at -80°C. | |
| Adenoviral - Titer (plaque assay) | $165 | $275 | 3 weeks | This requires at least 20 μl of purified virus. | |
| Adenoviral - Crude Lysate, 50 mls | $90 | Inquire | 2 weeks | The cell debris is removed, but Crude Lysate is NOT purified or concentrated. | |
| Lentivirus/ Retrovirus |
Lentiviral shRNA tester samples (5 × 3 ml of 1X virus) | $180 | Inquire | 2 weeks | PLEASE note the backbone of each construct, so the Vector Core can check the packaging generation. Each 3 ml tester prep requires 2.5 μg of purified proviral plasmid DNA. |
| Lentiviral/Retroviral - Concentrated Small Scale Prep (10 mls of 10X virus in DMEM or as requested) | $375 | $620 | 2 weeks | PLEASE note the backbone of each construct on the order form, so the Vector Core can check the packaging generation. Each small scale prep requires 70 μg of purified proviral plasmid DNA. This retroviral service requires the VSVG envelope. | |
| Lentiviral/Retroviral - Concentrated Large Scale Prep (100 mls of 10X virus in DMEM or as requested) | $920 | $1,515 | 2 weeks | PLEASE note the backbone of each construct on the order form, so the Vector Core can check the packaging generation. Each large scale prep requires 650 μg of purified proviral plasmid DNA. This retroviral service requires the VSVG envelope. For in vivo work, 100X concentration is typical. | |
| Lentivirus - Pre-made Standard Glycerol Stock (10 mls at 10X in DMEM) | $215 | $350 | same day | Pre-made stocks of popular lentiviral vectors at 107 TU/ml (pLL3.7dsRED, pLLEV GFP, pLLRSV GFP, pLLEV Luc, pGipZ Scrambled). Store at -80°C. | |
| Retrovirus - UNCONCENTRATED Small Scale Prep (100 mls of 1X virus). For ecotropic and amphotropic envelope pseudotypes. | $320 | $525 | 2 weeks | For use with MMLV/MSCV plasmids pseudotyped with ecotropic and amphotropic envelopes which are too fragile to concentrate without great loss. The unconcentrated small scale prep requires 100 μg of proviral DNA. | |
| Lentiviral/Retroviral Transduction | $95 | $155 | 1 week | Transduction of lentiviral constructs onto cells provided by the customer, or on A549 lung carcinoma cells provided by the Vector Core. | |
| Lentiviral/Retroviral Titer with Fluorescence | $165 | $275 | 1-2 weeks | 200 μl of virus is required. | |
| Lentiviral/Retroviral Titer with Antibiotic Selection | $250 | $415 | 2-3 weeks | 200 μl of virus is required. | |
| shRNA Library Clones | Ordering shRNA Library Clones | $21 per clone (if available in our library), or 50% off the cost when ordering a clone from the company plus a $40 order fee. | Not Available | 1-2 days for in-stock clones | The Vector Core currently stocks all pGipZ V2L and a few other lentiviral shRNA/shRNAmir clones. For clones not in-stock, we offer a cost sharing service for pGipZ V3L, pTripZ and pLKO.1 clones. pLKO.1 clones are offered from both Sigma and Open Biosystems. |
| Plasmid | Plasmid Midi Prep (~100 μg) | $60 | $100 | 1 week | |
| Plasmid Mega Prep (~1000 μg) | $365 | $605 | 1 week | ||
| Consultation | Consultation - 1 hour | $45 | Inquire | 1 week to schedule appointment | Having trouble planning/executing your project? Consult with the Vector Core manager Tom Lanigan for advice on how to use our vectors. |
| Custom Services | Inquire | Inquire | Do you require a service that is not listed on our order forms? E-mail Tom Lanigan, lanigant@umich.edu with what you require and we will customize a solution for you! |
* Discounts may be available for members of Centers that support the Vector Core (Cancer - 25%; GI Peptide - 50%).
