This primer pair is used to amplify the endogenous rat prolactin gene. Amplification will thus demonstrate that the DNA sample is a good substrate for PCR .

5' GCT TCT GAG CAA TGA CAC CA 3' 18 mer

5' ATT CCA GGA GTG CAC CAA AC 3' 18 mer

Size of expected amplification product is 391 base pairs.

Thermal cycler profile:

1. 95 degrees for 45 seconds

2. 95 degrees for 45 seconds
3. 55 degrees for 1 minute
4. 72 degrees for 30 seconds
5. Go To #2 30 times

6. 72 degrees for 10 minutes
7. Hold at 4 degrees
 

Gubbins EJ, Maurer RA, Lagrimini M, Erwin CR, Donelson JE. Structure of the rat prolactin gene. J Biol Chem. 1980 Sep 25;255(18):8655-62.