This primer pair is used test DNA for the presence of the neomycin gene.
The primers recognize sequences within the neo^r cassette of gene targeting vectors.

5' AGG ATC TCC TGT CAT CTC ACC TTG CTC CTG 3' 30mer

5' AAG AAC TCG TCA AGA AGG CGA TAG AAG GCG 3' 30mer

Size of expected amplification product is 492 base pairs.

Thermal cycler profile:

94 degrees C 30 sec
72 degrees C 120 sec

30 cycles

72 degrees C 10 min
 4 degrees C Hold