lacZ Primers

This primer pair is used to genotype transgenic mice that carry the beta galactosidase (lacZ) reporter gene. The primers recognize sequences within the beta galactosidase gene.

5' TTC ACT GGC CGT CGT TTT ACA ACG TCG TGA  3' 30mer

5' ATG TGA GCG AGT AAC AAC CCG TCG GAT TCT  3' 30mer

Size of expected amplification product is 364 bp.

Thermal cycler profile:

94 degrees C 60 sec
72 degrees C 120 sec

30 cycles

72 degrees C 10 min
 4 degrees C Hold


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Genotyping Transgenic Mice by PCR
Isolation of DNA from Mouse Tail Biopsies
Transgene Copy Standards
beta Globin Primers
neo Primers
beta galactosidase staining of mouse tissues
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