This primer pair is used to amplify the endogenous mouse beta-globin gene. Amplification will thus demonstrate that the DNA sample is a good substrate for PCR .

5' CCA ATC TGC TCA CAC AGG ATA GAG AGG GCA GG 3' 32mer

5' CCT TGA GGC TGT CCA AGT GAT TCA GGC CAT CG 3' 32 mer

Size of expected amplification product is 494 base pairs.

Thermal cycler profile:

94 degrees C 30 seconds
60 degrees C 90 seconds
72 degrees C 120 seconds

35 cycles

72 degrees C 10 min
 4 degrees C Hold

Konkel DA, Tilghman SM, Leder P. 1978. The Sequence of the Chromosomal Mouse beta-globin Major Gene: Homologies in Capping, Splicing and Poly(A) Sites. Cell 15:1125-1132.

 

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