Modified N5 Buffer Preparation


This modified buffer is designed increase yields of GC rich DNA or other "sticky" DNA from the Nucleobond column.
The buffer contains 50% formamide, 1.0 M KCl, 15% EtOH, 100 mM Tris-phosphate, pH 8.5.

Add 12.11  Tris base to 250 ml distilled water and dissolve
Add 74.55 g KCl to Tris solution
Autoclave this solution

Add 150 of absolute ethanol to cooled solution
Add 500 ml of formamide to solution
Adjust pH to 8.5 with phosphoric acid - do not overshoot pH
Adjust final volume with sterile distilled water to 1000 ml

Store at room temperature