Michaelis-Menten reaction; pseudo-first-order kinetics; flooding method; progress curves; rate constants; fitting procedure

The condition for pseudo-first-order kinetics in enzymatic reactions is independent of the initial enzyme concentration

The linearization of the Michaelis-Menten reaction by pseudo-first-order kinetics is revised. A phase-plane analysis allows the derivation of a new condition for its validity that is directly linked to the reaction efficiency, and contrary to widely …