Enzyme kinetics; Coupled enzyme assays; Michaelis-Menten reactions; Time-dependent slow manifold; Sisyphus manifold; Laelaps manifold; Differential-algebraic equation; Asymptotically autonomous vector field

The determination of a substrate or enzyme activity by coupling one enzymatic reaction with another easily detectable (indicator) reaction is a common practice in the biochemical sciences. Usually, the kinetics of enzyme reactions is simplified with …