Coupled enzyme reactions; Auxiliary enzyme assay; Slow manifold; Timescale separation; Singular perturbation analysis; Initial rate experiments; Reactant-stationary approximation; Schnell-Mendoza equation

A kinetic analysis of coupled (or auxiliary) enzyme reactions

As a case study, we consider a coupled (or auxiliary) enzyme assay of two reactions obeying the Michaelis-Menten mechanism. The coupled reaction consists of a single-substrate, single-enzyme non-observable reaction followed by another …