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Enzyme MechanismPlease note: Words in italics are the official PIBS affiliations of the indicated faculty. David P. Ballou, Ph.D. Mechanisms of biological redox reactions and reactions with oxygen, especially metalloproteins and flavoproteins; physical and chemical studies, including rapid reaction techniques and instrumentation development. Biological Chemistry. Biophysics.
James Bardwell, Ph.D. Protein folding catalysts, from
genetics to 3-D structures: role of molecular chaperones
and disulfide catalysts. Biophysics.
Biology. Cellular & Molecular Biology. Thomas Brock, Ph.D. Molecular and cellular aspects of cell-to-cell communication by polyunsaturated fatty acids, particularly in the contexts of immune defense and disease. Regulation of enzymes that convert fatty acids into alternative lipid mediators. Bioinformatics. Cellular & Molecular Biology. Internal Medicine Heather Carlson, Ph.D. Computer modeling of protein-ligand complexes. Interested in the basic biophysics of the process as well as drug design; also mine protein-ligand databases for proteomic information about ligand recognition. Biophysics. Bioinformatics. Dimitri Coucouvanis, Ph.D. Synthetic analogs for the active sites of metalloproteins. Biophysics. Chemistry.
David R. Engelke, Ph.D. Regulation of eukaryotic gene
expression at the levels of transcription and RNA
processing; catalytic and therapeutic RNA. Cellular & Molecular Biology. Biological Chemistry.
Carol A. Fierke, Ph.D. RNA Processing. Protein
Prenylation. Structure, Mechanism and Inhibition of
metalloenzymes and ribozymes. Protein engineering of
biosensors and biocatalysts. Biological Chemistry.
Biophysics.
Robert S. Fuller, Ph.D. Proteolytic processing,
protein localization, and macromolecular transport in the
secretory pathway; yeast cell biology; structure,
mechanism, and specificity of proteases. Biological
Chemistry. Cellular & Molecular Biology. Ari Gafni, Ph.D. Spectroscopic studies of protein structure; aging phenomena at the molecular level; protein folding mechanisms in vitro and in vivo; protein misfolding in aging and disease; mechanisms of amyloid generation and deposition in diabetes and Alzheimer's disease. Biological Chemistry. Biophysics. David Ginsburg, MD Molecular genetics of the blood coagulation system. Human Genetics. Cellular & Molecular Biology. Paul F. Hollenberg, Ph.D. Chemical carcinogenesis: metabolic activation and detoxication of carcinogens, mechanisms of carcinogen-activating enzymes, cytochrome P-450 and biological oxidations and peroxidations. Pharmacology. Neil Marsh, Ph.D. Enzymology: Structure and mechanism of coenzyme B12 and S-adenosylmethionine-dependent radical enzymes. Protein Design: synthesis of "Teflon" proteins - introducing new properties into proteins using fluorinated amino acids. Biophysics. Biological Chemistry. John Moran, Ph.D. Molecular analysis of human and mouse retrotransposons. Characterization of a novel retrotransposition mechanism. Studies of how transposable elements influence genomic diversity. Exploitation of human and mouse retrotransposons as genetic tools. Human Genetics. Alexander J. Ninfa, Ph.D. Reconstitution of signal transduction systems from purified components, structure/function analysis of signal transduction enzymes, protein crystallography. Characterization of protein kinases, phosphatases, and nucleotidy/transferases involved in signal transduction. Organization of the gene cascade controlling nitrogen assimilation in bacteria. Development of synthetic systems that perform useful functions. Biological Chemistry. Microbiology & Immunology. Patrick O'Brien, Ph.D. Biochemical, biophysical, and structural approaches to understanding mechanisms of human DNA repair. Biological Chemistry. Laura Olsen, Ph.D. Molecular and cellular biology of peroxisomes - peroxisome biogenesis and function. Molecular, Cellular & Developmental Biology. Yoichi Osawa, Ph.D. Formation of reactive intermediates such as nitric oxide, oxygen metabolites, and drug metabolites and their subsequent interactions with important cellular targets as potential mechanisms by which drugs, environmental pollutants, and certain pathological conditions lead to toxicity or disease. Pharmacology. Bruce Palfey, Ph.D. Mechanistic enzymology with a focus on flavoproteins involved in pyrimidine biosynthesis, tRNA maturation, inhibitor design, and structure/function relationships. Biological Chemistry. Vincent L. Pecoraro, Ph.D. Biological chemistry of manganese and vanadium; de novo design of metallopeptides. Biophysics. Chemistry. James E. Penner-Hahn, Ph.D. Structural characterization of metalloprotein metal sites; molecular mechanisms of photosynthesis; investigation of Zn biochemistry and the roles of Zn in development; EXAFS and X-ray absorption spectroscopy of proteins. Biophysics. Marc Peters-Golden, MD Cellular and molecular regulation of synthesis, as well as actions, of arachidonic acid metabolites (prostaglandins and leukotrienes) in inflammation, antimicrobial defense, and fibrosis. Immunology. Stephen W. Ragsdale, Ph.D. We are studying the microbial metabolism of one-carbon compounds (CO, CO 2 , methane) and xenobiotics (e.g., PCBs); oxygen sensing in the human carotid body; and the roles of metal ions in biology, including the mechanisms of nickel, B 12 , heme, and iron-sulfur enzymes. We use transient and steady-state kinetics, spectroscopy, and molecular biology to uncover mechanistic information. Biological Chemistry. Chemical Biology. Rudy J.
Richardson, ScD, DABT Mechanisms of neurological
disorders, stroke and autoimmune diseases and their
modulation by xenobiotics, genetics and age. Biomarkers
of xenobiotic exposure or disease. Interactions of
ligands with macromolecules using kinetics, molecular
modeling and mass spectrometry. Environmental
epidemiology. Scientific basis of risk assessment and
public health policy. Neuroscience. Gabrielle Rudenko, Ph.D.Determining the three-dimensional structure of proteins with important functions in the brain using x-ray crystallography, augmented with biochemical, biophysical as well as mutagenesis studies. Pharmacology. Life Sciences Institute JoAnn Sekiguchi, Ph.D. Mechanisms of DNA repair and how aberrant repair processes affect genomic stability, predisposition to cancer and immune system development. Human Genetics. Roseanne Sension, Ph.D. Ultrafast spectroscopic studies of enzyme mechanism. Biophysics. Chemistry. James A. Shayman, MD Mechanisms of cellular signaling by sphingolipid development of inhibitors for treating sphingolipid storage disorders. Pharmacology. Internal Medicine. Janet Smith, Ph.D. Structure-function studies of proteins using X-ray crystallography with an emphasis on complex enzymes and the replication proteins of flaviviruses and alphaviruses. Biological Chemistry. William L. Smith, Ph.D. Chemical processes underlying the biosynthesis and functions of prostaglandins. Current research is focused on understanding (a) the enzymology of prostaglandin endoperoxide H synthases (PGHSs) and (b) the regulation of PGHS gene expression. PGHSs are also known as "cyclooxygenases". Biological Chemistry. Yi Sun, Ph.D. 1) Mechanism of p53 tumor suppressor and SCF E3 ubiquitin ligase (for protein ubiquitination and degradation) in apoptosis induction and suppression, respectively; 2) Identification and validation of cancer targets in the p53 and E3 ubiquitin ligase signaling pathways for anti-cancer drug discovery. Cellular & Molecular Biology. Radiation Oncology. Roger Sunahara, Ph.D. Structure/function of the G-protein-coupled signaling cascade; molecular mechanisms of hormone receptor-mediated activation of G proteins; molecular mechanisms of activation and regulation of adenylyl cyclase. Pharmacology. Alice
Telesnitsky, Ph.D. Retrovirus molecular genetics,
reverse transcriptase structure-function studies, HIV-1
and murine leukemia virus genetic recombination and other
mechanisms of genetic variation, virus-host interactions
on the cellular level. Microbiology & Immunology.
Cellular & Molecular Biology. John Tesmer, Ph.D. Structural studies of heterotrimeric G protein signaling using X-ray crystallography and other biophysical techniques. Life Sciences Institute, Pharmacology. Raymond C. Trievel, Ph.D. Structural and functional studies of enzymes that catalyze post-translational modifications of proteins, especially those involved in transcriptional regulation and chromatin remodeling. Biological Chemistry. Nils G.
Walter, Ph.D. Folding and function of non-coding RNA; structural and single molecule RNA enzymology; ribosome and spliceosome function by single molecule microscopy; single molecule systems biology. Biophysics. Cellular & Molecular Biology.
Thomas E. Wilson, MD, Ph.D. DNA double-strand break
repair mechanisms and their contribution to the
chromosomal instability of cancer. Molecular and
Cellular Pathology. Cellular & Molecular Biology. Matthew J. Wishart, Ph.D. Mechanisms of action for protein- and lipid-phosphatases; STYX / dead-phosphatase adapters and phosphorylation-mediated signaling; molecular mechanisms of mammalian reproduction. Molecular & Integrative Physiology Robert Zand, Ph.D. Studies of posttranslational modification in human normal and multiple sclerosis myelin basic protein. Also studies of demyelination in genetically modified mice. These studies involve spectroscopic methods such as UV-Vis, IR, CD, Mass Spectrometry, and NMR.. Biological Chemistry. Biophysics. Erik R. P. Zuiderweg, Ph.D. Determination of three-dimensional structures of biomolecules and complexes of biomolecules in solution, using multidimensional heteronuclear NMR spectroscopy; NMR studies of biomacromolecular conformation and dynamics in solution, concentrating on methodology developments and on applications involving domains of Hsp-70 chaperone and metal binding proteins such as matrix-metalloproteinase proteins. Biophysics. Biological Chemistry.Chemistry. |
