Study consisting of n=300 women presenting to County Health
Department STD clinic in Pittsburgh, PA.2
All women underwent swab sampling of vaginal introitus, urethra,
and cervix for PCR, EIA and culture. First 5cc of clean catch
specimen used for urine PCR.
n=200 women instructed on self-collection of urine and vaginal
Study used an expanded gold standard – any positive culture
or PCR. If culture was negative but PCR positive from any
site, an alternate PCR probe was used to confirm positivity.
Prevalence of chlamydia in this population was 37/300 patients
PCR on cervical specimens showed sensitivity of 86% vs. 84%
by culture and 68% by EIA. PCR on urethral specimens
showed 75% sensitivity vs. 54% by culture. PCR on vaginal
introitus specimen showed sensitivity of 92%.
In the n=200 sub-population performing self-collection techniques,
PCR on introitus specimens yielded sensitivity of only 81%,
and on clean-catch urine – 73%.