Bioluminescent signals from PC-3^Luc cells are precise indicators for the locations of metastatic lesions. Grey scale images with identically scaled pseudo-color overlays represent rotational views of the same mouse from ventral (A), right lateral (B), dorsal (C), and left lateral orientations at week 7 and aid in identifying tumors at necropsy (E-H). Photon signals at midline in (A, B) coincide with enlarged right adrenal gland (E) shown with attached uninvolved kidney as gray scale (left panel) and pseudo-color overlay (right panel) images. Leg bone emissions (F, left panel) correlate with left hind limb signals in whole animal imaging (A, C, D). White arrowheads in autoradiographic image (F, right panel) indicate osteolytic lesions characteristic of PC-3 cells. Lumbar lymph node (G) and mandible (H) emissions represent lower spine (C) and jaw (A, B) signals respectively. Note that due to poor quality of the isolated mandible for this mouse, (H) is derived from another mouse with similar bioluminescence. Signals from residual cells at the site of intracardiac injection are visible in (A), and emissions at the left scapula (C, D) represent metastatic lymph nodes. Bioluminescent signals from PC-3^Luc cells are precise indicators for the locations of metastatic lesions. Grey scale images with identically scaled pseudo-color overlays represent rotational views of the same mouse from ventral (A), right lateral (B), dorsal (C), and left lateral orientations at week 7 and aid in identifying tumors at necropsy (E-H). Photon signals at midline in (A, B) coincide with enlarged right adrenal gland (E) shown with attached uninvolved kidney as gray scale (left panel) and pseudo-color overlay (right panel) images. Leg bone emissions (F, left panel) correlate with left hind limb signals in whole animal imaging (A, C, D). White arrowheads in autoradiographic image (F, right panel) indicate osteolytic lesions characteristic of PC-3 cells. Lumbar lymph node (G) and mandible (H) emissions represent lower spine (C) and jaw (A, B) signals respectively. Note that due to poor quality of the isolated mandible for this mouse, (H) is derived from another mouse with similar bioluminescence. Signals from residual cells at the site of intracardiac injection are visible in (A), and emissions at the left scapula (C, D) represent metastatic lymph. (Cancer Biology & Therapy 2;6, 17-17 November/December 2003)