Molecular Phenotyping Core
Director Subramaniam Pennathur, MD, PhD Medical School
Co-Directors Robert Kennedy, PhD School of Letters and Science, Chemistry
The Molecular Phenotyping Core will provide analytical tools to the MMOC investigators to permit structural identification and quantification of metabolites and perform functional metabolic studies in in vitro systems. In addition to providing instrumental infrastructure, the Core staff will provide consultation and collaboration to apply metabolomic platforms in nutrition and obesity research.
The Core consists of 3 Laboratories
- Directed Metabolomics Laboratory
- Unbiased Metabolomics Laboratory
- Lipomics Laboratory
Services provided by the Molecular Phenotyping Core include:
- A variety of metabolomic platforms for analysis of biospecimens derived from murine, rodent, human and plant sources as well as from primary and established cell lines and subcellular fraction.
- Assistance to Center Investigators in the experimental design and techniques to generate samples for metabolomic analysis.
- Facilitation of interaction between investigators with the Integrated Biostatistics and Informatics Core to integrate metabolomic and other molecular phenotyping into clinical, translational and population studies.
- Expertise and advice in the design, execution and analysis of studies using heavy isotope flux experiments for assessment of intermediary metabolism utilizing the metabolomic platform.
- Maintainance of functional metabolomic analysis systems for use in MMOC-related studies.
- Provide infrastructure, expertise and training for in vitro analysis of cellular and mitochondrial metabolism via the use of SeaHorse and Oxitherm technology.
- Develop new techniques and acquire new technologies for molecular phenotyping in accordance with the needs of the MMOC investigators.
- Provide training in technologies related to molecular phenotyping.
