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Instrumentation & Reservations
Microscopy & Image Analysis
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Microscopes - BSRB
Digital Imaging
Instrumentation - NCRC
Other Instrumentation
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Deltavision-RT Live
Cell Imaging System Deltavision image restoration system for live cell imaging. Widefield fluorescence offers two filter sets, one for fixed samples, one for fluorescent proteins. Fully programmable stage lets the user select numerous points for visitation over lengthy live cell imaging. Deconvolution and image capture with Applied Precision's SoftWorx imaging software. |
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Olympus BX-51 upright light microscope (A) Research grade optics include 4,10,20,40X dry; 60 & 100 X oil immersion. Standard fluorescence reflector cubes (DAPI, 488, TRITC) with full complement of filters for DIC imaging. Olympus DP-70 camera and software allows users to overlay fluorescent images pixel for pixel as well as overlay DIC-fluorescence. Scale bars can be burned into captured images in a wide array of user selectable image sizes. |
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Olympus BX-51
upright light microscope (B) For brightfield, differential interference contrast (DIC), widefield fluorescence (350, 488, 543 nm), microscopy. Image capture is performed electronically with an Olympus DP-70 high resolution digital camera |
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Leica MXFL III
stereo fluorescent microscope Leica MZFL III Stereo/Dissecting microscope has three brightfield input illuminators plus fluorescence. Can be operated in either brightfield or darkfield modes. Olympus DP-70 digital camera and software for static, time lapse, or video rate image capture. Auxiliary heated stage is available. Interchangeable objective lenses. |
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Leica DMIRB inverted
microscope Leica DMIRB inverted fluorescence microscope for live cell imaging. Heated stage allows for short-term live cell imaging in either standard fluorescence, DIC, or phase contrast modes. Olympus DP-30 B&W camera and software has time lapse or video rate digital imaging. |
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AMRAY 1910 Field Emission Scanning Electron Microscope (FEG-SEM)
AMRAY 1910 field emission scanning electron microscope with Robinson backscattered detector. Offers high resolution at low accelerating voltages. Digital image capture offered through SemTech Solutions X-Stream Image capture software.
Reserve time on this instrument |
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Philips CM-100 transmission electron microscope (TEM) Equipped with an computer controlled compustage and a high resolution (2K X 2K) digital camera. AMT software drives the digital camera and provides on screen live viewing . Scale bars are automatically created for incorporation into saved images as a separate operator function if desired. Download
the Operating Manual |
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Zeiss
LSM 510-META Laser Scanning Confocal Microscope Mounted on a Zeiss Axiovert 100M inverted microscope. This instrument is equipped with four lasers offering a wide range of excitation wavelengths. Coherent Enterprise laser for UV (351,364 nm), Argon laser for FITC/GFP (458, 488, 514 nm), Helium Neon 1 laser for Rhodamine,Texas Red,Cy3 (543 nm), and Helium Neon 2 laser for Cy5 (633 nm). Using the META system, the researcher can easily separate highly overlapping emission peaks of
Reserve time on this instrument |
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Olympus FluoView 500 Laser Scanning Confocal Microscope Olympus Fluo-View 500 confocal microscope system. Four laser system includes 405 diode, multi line Argon, HeNe green and HeNe red. Transmitted light detector for DIC image capture. Pathology Devices stage top incubator is available for live-cell imaging controlling temperature and CO2. System is on an IX-71 inverted platform with high NA objectives: 10, 20 dry, 40X oil, 60X oil, 60X water immersion, and 100X oil.
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Arcturus "Veritas" Laser Capture Microdissection System This system houses two lasers for the microdissection of individual cells or larger areas of tissue for proteomic and genomic analysis. |
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Zeiss Apotome Upright widefield fluorescent and brightfield microscope offering Zeiss' patented grid scanning algorithm for structured illumination. Wide array of high numerical aperture objectives from 10X to 100X oil immersion. Fluorescent filters and exfo-illumination system for DAPI, GFP, TRITC, and Ds-red/Cy5. High resolution axiocam camera system allows for user selectable multiple image formats. Motorized z-stage for in-depth data set collection. Reserve time on this instrument |
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Leica Inverted SP5X Confocal Microscope System with 2-Photon FLIM Leica SP5X confocal on an inverted DMI 6000 stand offering AOBS tunable filters for spectral scanning in both excitation and emission pathways. Supercontinuum "white-light laser" can be tuned to offer user selectable excitation wavelengths between 470-670 nm. 405 Diode for UV excitation plus a multi-line argon laser for GFP/FITC. Spectra-physics "Mai-Tai" 2-photon laser tunable from 640-1040 nm. Picoquant SMD fluorescent lifetime imaging module (FLIM) is integrated into the Leica software. Avalanche diode detectors for superior sensitivity and resolution. Remote workstation with full Leica software along with Autoquant for deconvolution. Fully programmable motorized stage in x,y, and z. Mark, Find, and Tile operation is included in the software for image reconstruction. Tokai-Hit environmental chamber is included for live-cell imaging controlling temperature, CO2, and humidity. |
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Leica Upright SP5X Confocal Microscope with 2-Photon Leica SP5X confocal on an upright DMI 6000 stand offering AOBS tunable filters for spectral scanning in both excitation and emission pathways. Supercontinuum "white-light laser" can be tuned to offer user selectable excitation wavelengths between 470-670 nm. 405 Diode for UV excitation plus a multi-line argon laser for GFP/FITC, photoactivation, photobleaching. Coherent "Chameleon" 2-photon laser with a tuning range of 690-1040 nm. Designed for 'in-vivo" imaging with three separate interchangeable objective turrets. Resonant scanner offers high scan speeds with no loss of resolution. Simultaneous imaging of up to 8 lines. Environmental chamber wraps around the instrument to maintain temperature, CO2, and humidity for lengthy time lapse imaging. |
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Nikon E-800 Upright with Widefield Microscope Used for routine fluorescence or brightfield including DIC optics and filters. Image acquisition is through an Olympus DP-71 digital camera. Full range of the normal fluorescent filters excited by a 100W mercury HBO lamp. |
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Diode based laser system for 405,488,561, and 640 excitation. Operates with Nikon's Elements software. |
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HP Scanjet IIcx flatbed scanner for image acquisition and conversion of paper documents to electronic format (OCR) |
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Pit PC1 (Windows XP) Pit PC 1 image analysis computer. This is a remote workstation software for Zeiss confocal, Olympus confocal, and Metamorph image analysis software |
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Cilia (Windows XP) Microsoft Office (Word, PowerPoint, Excel), Adobe Photoshop, Adobe Illustrator, Fluoview, SoftWorx, ImageJ, Metamorph, Volocity |
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Golgi (Mac OSX) Adobe Photoshop, Adobe Illustrator, Microsoft Office (Word, PowerPoint, Excel), Volocity |
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Synapse Imaging Computer |
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Leicia 3050S Cryostat |
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Leica DMIRE Leica DMIRE inverted fluorescent Microscope with DIC and programmable Stage |
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Nikon A-1 Spectral Confocal |
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Nikon E-800 Widefield Microscope Nikon E-800 Upright Microscope Widefield fluorescence and brightfield with DIC |
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N-1 Imaging - Imaris N-1 Imaging computer with Imaris, LAS-AF Lite for Leica data-sets Autoquant and Picoquant (FLIM)y |
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N-2 Imaging Computer |
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Olympus SZX16 |
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2-Photon Confocal with FLIM & FCS |
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Zeiss Axioplan-2 |
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Polaron E5100 Sputter Coater for gold or gold/palladium coating |
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Denton DV-502 high vacuum evaporator |
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H&E Stain Station |
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Reichert Ultracut-E ultramicrotomes (3)
Instrument #1 –
Reserve time on this instrument |
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Leica 2235 rotary paraffin microtome |
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Leica 2155 rotary paraffin microtome. |
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Leica 3050S cryostat |
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Microm 550 cryostat for sectioning frozen tissue. Disposable blade holder. Automatic or manual operator modes. |
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Tissue-Tek paraffin tissue embedding station. |
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Leica ASP 300 paraffin tissue processor. Has multiple program lengths to suit various tissue types and sizes. |
