Host & Bacterial Adaptation to Intracellular Infection
Our laboratory explores the interaction of intracellular bacterial pathogens with their host cells. We primarily study the Gram-positive bacterium Listeria monocytogenes, a facultative intracytosolic pathogen, investigating mechanisms of adaptation to the host cell environment. In addition, we use L. monocytogenes and other bacterial pathogens as tools with which to explore host mechanisms that regulate infection and innate immune signaling.
Projects in the lab include:
Defining host:pathogen interactions that regulate infection by the intracellular bacterial pathogen Listeria monocytogenes
Elucidating pathways of bacterial metabolism that enable Listeria monocytogenes to replicate robustly in the mammalian cytosol, facilitating infection and cell-to-cell spread.
S. Cassidy, J. Hagar, A. Paras, T. Devi-Kanneganti, G. Nunez and M. O'Riordan. Membrane damage during Listeria monocytogenes infection triggers a caspase-7 dependent cytoprotective response. PLoS Pathogens. In press 2012.
K. Burkholder, J. Perry, N. Donato, C. Wobus and M. O'Riordan. A small molecule deubiquitinase inhibitor increases localization of iNOS to the macrophage phagosome and enhances bacterial killing. Infection & Immunity. 2011 Dec;79(12):4850-7.
A. Radtke, K. Anderson, M. Davis, J. Swanson, M. DiMagno and M. O'Riordan. Listeria monocytogenes exploits CFTR-mediated chloride transport to escape the phagosome. Proc Natl Acad Sci U S A. 2011 Jan 10.
Y. Sun and M. O'Riordan. Branched chain fatty acids promote Listeria monocytogenes intracellular infection and virulence. Infection & Immunity. 2010 Nov;78(11):4667-73. Epub 2010 Sep 7.
K. Keeney, L. Colosi, W. Weber and M. O'Riordan. 2009. Generation of branched chain fatty acids through lipoate dependent metabolism facilitates intracellular growth of Listeria monocytogenes. J. Bacteriology. Apr;191(7):2187-96.
M. O'Riordan, L. Bauler, F. Scott and C. Duckett. 2008. Inhibitor of Apoptosis (IAP) Proteins in eukaryotic evolution and development: a model of thematic conservation. Developmental Cell. Oct;15(4):497-508.
L. Delbridge Bauler, C. Duckett and M. O'Riordan. 2008. XIAP regulates cytosolic-specific innate immunity to Listeria infection. PLoS Pathogens. Aug 29;4(8):e1000142.
A. Radtke and M. O'Riordan. 2008. Homeostatic maintenance of pathogen-containing vacuoles requires TBK1-dependent regulation of aquaporin-1 . Cellular Microbiology. Jul 8 [Epub ahead of print].
K. Keeney, J. Stuckey and M. O'Riordan. 2007. LplA1-dependent utilization of host lipoyl-peptides enables Listeria cytosolic growth and virulence. Molecular Microbiology. Oct 1 [Epub ahead of print].
A. Radtke, L. Delbridge S. Balachandran, G. Barber and M. O'Riordan. 2007. TBK1 protects vacuolar integrity during intracellular bacterial infection. PLoS Pathogens. Mar 2;3(3):e29.