5641 Medical Science Bldg. II
1150 West Medical Center Dr.
Ann Arbor, Michigan 48109-5620
Telephone (734) 763-3531
FAX (734) 764-3562

Faculty

Cheong-Hee Chang

Professor
Ph.D, University of Virginia, 1990
heechang@umich.edu




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Regulation of innate-like CD4 T cell development and function

The research program in my laboratory has been focusing on investigating and understanding the molecular mechanisms that govern the adaptive immune function. In particular, we are studying CD4 T cell development and effector functions.

It is well accepted that the immune system of humans is similar to that of mouse and therefore mouse models are commonly used to study immune responses and diseases. However, the two exhibit differences in CD4 T cell development. Conventional understanding of CD4 T cell development is that the MHC class II molecules on cortical thymic epithelial cell (TEC) are necessary for selection, as shown in mouse models. Clinical data, however, show that hematopoietic stem cells reconstitute CD4 T cells in patients devoid of MHC class II. The difference observed in humans can be explained by our discovery that the CD4 compartment is efficiently reconstituted by MHC class II expressing thymocytes, demonstrating a novel thymocyte-driven pathway of CD4 T cell selection. Thus, both epithelial cells and thymocytes support human CD4 T cell development and, as a consequence, two CD4 T cell populations coexist in humans.

We showed that thymocyte-selected CD4 (T-CD4) T cells but not epithelial cell-selected CD4 (E-CD4) T cells produce both Th1 and Th2 cytokines immediately after stimulation, suggesting that they could be potent helper T cells. In addition, T-CD4 T cells do not require Stat6 to express the IL-4 gene and the IL-4 locus is already remodeled upon positive selection suggesting that T-CD4 T cells have received distinct signals by thymocyte-thymocyte interaction during selection. Surprisingly, T-CD4 T cells inhibit the generation of effector CD4 T cells or memory CD8 T cells upon Helicobacter pylori or Listeria monocytogenes infection, respectively. Unlike E-CD4 T cells, CD25-Foxp3- T-CD4 T cells suppress immune responses mediated by CD4 and CD8 T cells. Taken all together, T-CD4 T cells represent a unique T cell population and understanding of their development and function bears high significance.

Currently, 4 different projects are ongoing all of which address T-CD4 T cell development and function.

(1) Study of signaling pathways that regulate T-CD4 T cell selection, which in turn controls the function
(2) Investigation of the mechanisms by which T-CD4 T cells mediate immune suppression
(3) Research of human T-CD4 T cells regarding their function during immune responses
(4) Examination of T-CD4 T cells' role during viral infection in infants

Selected Publications:

Li, W., M. G. Kim, T. S. Gourley, D. Sant'Angelo, and C.-H. Chang. 2005. An Alternate Pathway for CD4 T cell Development: Thymocyte-Expressed MHC Class II Selects a Distinct T cell Population.  Immunity. 23:375.

Li, W., M. H. Sofi, N. Yeh, S. Sehra, B. P. McCarthy, D. R. Patel, R. R. Brutkiewicz, M. H. Kaplan, and C.-H. Chang. 2007. Thymic selection pathway regulates the effector function of CD4 T cells. J. Exp. Med. 204:2145. (Highlighted by the JEM and Nat Imm Rev).

Li, W., H. Sofi, S. Rietdijk, N. Wanag, C. Terhorst and C.-H. Chang. 2007. The SLAM-Associated Protein Signaling Pathway Is Required for Development of CD4+ T Cells Selected by Homotypic Thymocyte Interaction. Immunity 27:763–774 (Highlighted by Nat Imm Rev)

JiHoon Chang, Steven Kunkel and Cheong-Hee Chang. Negative regulation of MyD88-dependent signaling by Interleukin-10 in dendritic cells. PNAS. 2009. 106:18327-32

M. Hanief Sofi, Zhiping Liu, Lingqiao Zhu, Qiao Yu, Mark H. Kaplan and Cheong-Hee Chang. Regulation of IL-17 expression by the developmental pathway of CD4 T cells in the thymus. Mol. Immunol. 2010. 47:1262-1268.

JiHoon Chang, Timothy J. Voorhees and Cheong-Hee Chang. Protein Phosphatase 2A is a Negative Regulator of Interleukin-23 Expression in Dendritic Cells. PNAS. 2010. 107:8340-8345.

M. Hanief Sofi, Zhiping Liu, Lingqiao Zhu, Qiao Yu, Mark H. Kaplan and Cheong-Hee Chang. Regulation of IL-17 expression by the developmental pathway of CD4 T cells in the thymus. Mol. Immunol. 2010. 47:1262-1268.

M. Hanief Sofi, Yu Qiao, K. Mark Ansel, Masato Kubo, Cheong-Hee Chang. Induction and maintenance of IL-4 expression are regulated differently by the 3'enhancer in CD4 T cells. J. Immunol. 2011. 186: 2792-2799