Michigan Gastrointestinal Peptide Research Center

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Protein Identification and Localization Core

Facilities

The protein identification component of the PIL Core is adjacent to the National Resource for Proteomics and Pathways (NRPP) and shares some resources and personnel including the Director, Dr. Andrews.  The NRPP UM laboratory occupies approximately 800 square feet (rooms 1188 and 1195 of North Ingalls Building) of wet laboratory space and includes an ultracentrifuge, high-speed preparative centrifuge, IEF and 2D Gel equipment, gel scanner, cell culture equipment, HPLC, PCR, apparatus for organic synthesis, and other typical laboratory equipment.

The protein localization component of the PIL Core is located in the laboratories of Drs Williams and Ernst while the confocal and electron microscopes are located in the MIAC core of the MDRTC and in the MIL. Dr Williams’ lab is located on the 7th floor of Medical Sciences II. It contains general purpose equipment, a tissue culture room, a Nikon inverted fluorescence microscope with a digital camera and a computer workstation running Metamorph software for image analysis. Dr Ernst’s laboratory of about 200sq ft is located on the 3rd floor of the Biomedical Sciences Research Building (BSRB) and is devoted to the support of this core. It houses general purpose equipment plus a new Leica cryostat and a Reichert Ultracut E ultramicrotome with associated glass knifemaker and diamond knives. Bradley Nelson, the Research Assistant directed by Dr. Ernst who carries out fixation, embedding and sectioning, is located in this laboratory. The MIL which contains several confocal microscopes and the EM is located in the basement of the BSRB. The MIAC core of the MDRTC which contains confocal and widefield fluorescence microscopes is located on the 2nd floor of the BSRB but in early 2010 will move to the newly constructed Brehm Center for diabetes research located about 5 blocks away from MSII and the BSRB. Most GI Center members are located in the Medical Sciences Complex and the BSRB although some are in the Life Sciences Institute or will be in the new Brehm Center.

 

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