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Protein Localization, Identification and Folding Core
Services
Facilities and Resources
Discounts

In Vivo Animal and Human Studies Core

Molecular Biology Core

Microbiome and Metabolomics Core

Recharge Rates

The Image Analysis component of the PLIF Core maintains its own recharge system where the income is used to cover part of the salary of the morphology technician Mr. Nelson. Work for non-Center members is only done on a time-available basis and at a 50% higher amount for technician time (since the Core provides 50% of the technician, Mr. Nelson’s, salary). The Center member rates are summarized below.


The Proteomic facilities are available at a highly subsidized rate as they do not include instrument service contract costs. GI Center members can be approved for up to $1000 of these recharges (annually) billed directly to the GI Center. The fee structure is displayed in the table below.


The Protein Folding Diseases Initiative Resources, several services are available (summarized below). The technician who is supported by the PFD initiative is contacted directly by phone or email. Antibody aliquots or tissues are typically provided within 2 business days of making the request. If a particular antibody on the list is not available, the technician will order it from the vendor (with express shipping) and inform the investigator when it arrives. No more than one aliquot per antibody is provided to any given investigator. The PFD has not had to set limits on how many antibodies can be tested by a given investigator, but limits (e.g., no more than 5 per month per investigator) may be set if warranted. The antibodies are purchased by the PFD Initiative.

 The prices listed below are subject to change.

Imaging Services:

Core Service

Cost

Cryostat Usage

$4/hour; cryostat instruction – free

Immunohistochemistry Pilot

Free; member provides primary antibody

Immunohistochemistry service

$30/hour

EM Fixation and Resin Embedding

$200 first sample, $20 for additional

Cryosectioning, Thin sectioning, EM analysis and other technical work

$30/hour

Live Cell Imaging

Consultation free; microscope time at usual rate

Other work is carried out using instruments in Cores maintained by the Medical School or its Departments and Centers. Their current fee rates (not a complete list)

GI Center users can be approved for up to $500 of these recharges billed directly to the GI Center

Olympus Fluoview 500 Confocal Microscope

$27/hour MIL; $30/hour MIAC

Nikon A1 Confocal with perfect focus and environmental control

$30/hour MIAC

Zeiss LSM-510 Confocal

$43/hour MIL

Leica TCS SP5

$30/hour MIAC

Leica SP5 Inverted multiphoton with FLIM

$30/hour MIL

JEOL 1400-plus TEM

$24/hour MIL

Olympus BX-51 A Photomicroscope

$12/hour MIL

Computer Imaging Workstations

$8/hour MIL; $7.50/hour MIAC

Ultramicrotome

$5/hour MIL

Laser Caption Microdissection

$5/hour; $125 Training

Slide Scanner

$10/hour

Proteomic Services:

Core Service

Description

Cost (USD)

Simple Protein Identification

Includes digestion, 90 min LC-MS/MS analysis and Database search

158

Relative Quantitation (e.g. TMT-based)

Includes digestion, TMT-labeling, 90 min LC-MS/MS analysis and Database search

501

Phospho-enrichment Analysis

Includes digestion, phospho-peptide enrichment, 90 min LC-MS/MS analysis and Database search

501

Add-on Services

 

 

Extended gradient

3 h LC-MS/MS analysis

94

2D-LC

Fractionation followed by 90 min LC-MS/MS analysis (per fraction)

106

Protein Folding Diseases Initiative Resources:

Resources

Description

Cost (USD)

Antibody library

85 antibodies to ER stress, and other protein folding related pathways. An aliquot (typically 20 µL or ~20 µg, depending on antibody, is provided)

No charge

Mouse tissue library

GI and non-GI tissues from several commonly used mouse strains are available (typically 0.1-1.0 gram, stored in an ultrafreezer, and in some cases embedded fresh frozen tissues are available for immune staining)

No charge

Genetic mouse models

Breeding pairs are provided

No charge

Pilot program

Pilot grants (up to $50,000) for collaborative projects between PFD and UMGRC investigators

Not applicable

 

 

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