The Biomechemical Nuclear Magnetice Resonance Core provides support and consultation in the planning, implementation and analysis of experiments performed on a state-of-the-art high field 500 MHz NMR spectrometer.
Facility Personnel
Henry I. Mosberg, PhD, Director (him@umich.edu)
Scott Woehler, PhD, Spectroscopist (swoehler@umich.edu)
Address
College of Pharmacy
1553 C.C. Little Building, Box 1065
University of Michigan
Ann Arbor, Michigan 48109-1065
Phone 734-763-9284
Instrumentation
The state-of-the-art Bruker Avance DRX-500 NMR spectrometer with field gradients has an 11.75 Tesla field strength magnet, a 1H NMR frequency of 500 MHz and a 13C NMR frequency of 125.7 MHz. Multinuclear capabilities allow the observation of most NMR active nuclei (e.g. 1H, 2H, 13C, 14N, 15N, 17O, 19F, 31P, 57Fe, etc.). It is a "research grade" instrument capable of performing all of the solution NMR techniques commonly reported in the literature. Commonly performed experiments include simple spectra, T1 and T2 relaxation experiments, solvent suppression, selective decoupling and nOe experiments, as well as two-dimensional (2D) COSY, NOESY and HETCOR (also called CSCM or XHCORR). Other experiments required by the user can be implemented with the aid and supervision of the spectroscopist.
Scheduling and Sample Preparation
- Training: In most cases the spectroscopist will train investigators in the use of the NMR and provide expertise and troubleshooting of NMR instrumental problems as needed. In a few cases it may be necessary for the spectroscopist to perform the experiment. The decision will be made at the discretion of the spectroscopist. An additional fee is charged in the latter case.
- Instrument Use: Use of the NMR is scheduled by the spectroscopist. Please notify the spectroscopist of your instrument needs as far in advance as possible. Although instrument time is occasionally available on short notice, investigators should not rely on immediate access. Priority will generally be given to users who run their own experiments.
- Sample Preparation: NMR sample preparation is the responsibility of the user. It is also the user's responsibility to provide their own NMR tubes, deuterated solvents and pipets. The NMR tubes should be of high grade. Contact the manager for minimal NMR tube specifications and for advice on sample preparation.
Fees
University of Michigan Investigators $10 per hour
If Spectroscopist Performs Experiment $15 per hour
All Other Users $64 per hour
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