Hybridoma
The Hybridoma Core research laboratory is jointly supported by the MDRTC and by the University of Michigan Rheumatic Diseases Core Center.
The Hybridoma Core generates somatic-cell hybrids (hybridomas) that produce monoclonal antibodies of desired specificity. Since its establishment in 1980, the Hybridoma Core has produced monoclonal antibodies against a wide variety of lymphocyte surface antigens, tumor cell antigens, purified proteins, hormones, hormone receptors and recombinant proteins.
Services
Services include:
- immunization of mice
- fusion of B lymphocytes with myeloma cells to create hybridomas
- subcloning and cryopreservation of hybridomas
- antibody isotyping and
- production of ascites in mice
Consultation/Collaboration
Consultation is available from the Hybridoma Core Directors on the design of immunization strategies and screening assays to ensure efficient detection of the desired monoclonal antibodies. The majority of hybridomas produced in the Core are of murine origin, but rat, hamster and human hybridomas also have been produced.
The Core also provides collaborative and consultative services in conjunction with the MDRTC Cell & Molecular Biology Core on phage display libraries to assist investigators who may wish to select recombinant antibody-like reagents. Through these initiatives, the Hybridoma Core will remain on the cutting edge of monoclonal antibody technology, and continue to provide optimal service to MDRTC investigators.
Contact
Phone: 734-763-9271
E-mail: hybridoma@umich.edu
Lab: Room 3556 MSRB II
Office: Room 3560E MSRB II
University of Michigan Hybridoma Core Facility
Room 3556 MSRB II
1150 W. Medical Center Drive, Box 0688
University of Michigan
Ann Arbor, Michigan 48109-0688
Key Personnel
James R. Baker, Jr., M.D., MDRTC Core Director
jbakerjr@umich.edu; 734-936-5634David A. Fox, M.D., RDCC Core Director
dfox@umich.edu; 734-936-5566Elizabeth M. Smith, M.S., Assistant Director
emsmith@umich.edu; 734-763-9271
