Peptide and Proteomics
The goal of the Peptide and Proteomics Core is to enhance and stimulate
research in diabetes, its complications and related endocrine and metabolic
disorders by MDRTC investigators by providing the broadest range of resources
for the analysis of peptides and proteins. This core provides assistance
with peptide synthesis, including multiply labeled, cyclic peptides and
peptides with posttranslational modifications. N-terminal sequence analysis
is offered for protein characterization as is Circular Dichroism Spectroscopy
and state-of-the-art technology in protein analysis such as routine identification
of proteins by mass spectrometry. Proteomic services include proteome mapping,
protein expression profiling, protein complexes, and post-translational
modifications. Technologies offered include 1) protein expression profiling
using isotope enrichment, ion statistics, and two dimensional gel electrophoresis;
2) phosphorylation site mapping as well as mapping of other post-translational
modifications; 3) MuDPIT analyses; 4) 1D and 2D capillary HPLC; 5) bioinformatics
support and training; 6) tandem mass spectrometry; and 7) proteome informatics.
The Core maintains an information management system for proteomics, providing
an environment for investigators to access and curate their data.
- Peptide Synthesis Services:
- Linear Custom Peptides
- Peptides with Posttranslational Modifications or Unusual Amino Acids
- Labeled Peptides
- Peptide Libraries
- Cyclic Peptides
- Peptides for Immunological Applications
-
Peptide Purification and Quality Control
- Protein Mass Spectrometry Services:
- Molecular Weight Determination of Proteins
- Protein identification by LC-MS/MS
- Peptide Mass Fingerprinting
- Characterization and Identification of Posttranslational Modifications
- Other Protein Analysis Services:
- Circular Dichroism Spectroscopy
Recharge Rates
MDRTC investigators receive a 75 percent discount on the standard recharges of the Biomedical Research Core Facilities Protein Structure Facility. Usage by MDRTC investigators will be monitored and if it appears that usage will exceed the support level, the discount rate will be changed appropriately or a cap will be placed on usage by individual investigators.
For further information, please go to the Biomedical
Research Core Facilities Protein Structure Facility Web site or the
Michigan Proteome Consortium.
Key Personnel
Philip C. Andrews, PhD, Director, Peptide and Proteomics Core
andrewsp@umich.edu; 734 763-3130Henriette Remmer, Ph.D., Co-director, Peptide and Proteomics Core,
hremmer@umich.edu; 734-763-6285
