Peptide and Proteomics
The goal of the Peptide and Proteomics Core is to enhance and stimulate
research in diabetes, its complications and related endocrine and metabolic
disorders by MDRTC investigators by providing the broadest range of resources
for the analysis of peptides and proteins. This core provides assistance
with peptide synthesis, including multiply labeled, cyclic peptides and
peptides with posttranslational modifications. N-terminal sequence analysis
is offered for protein characterization as is Circular Dichroism Spectroscopy
and state-of-the-art technology in protein analysis such as routine identification
of proteins by mass spectrometry. Proteomic services include proteome mapping,
protein expression profiling, protein complexes, and post-translational
modifications. Technologies offered include 1) protein expression profiling
using isotope enrichment, ion statistics, and two dimensional gel electrophoresis;
2) phosphorylation site mapping as well as mapping of other post-translational
modifications; 3) MuDPIT analyses; 4) 1D and 2D capillary HPLC; 5) bioinformatics
support and training; 6) tandem mass spectrometry; and 7) proteome informatics.
The Core maintains an information management system for proteomics, providing
an environment for investigators to access and curate their data.
Recharge Rates
Charges for U-M-MDRTC investigators for all services are reduced by 75%. For a list of recharge rates for Protein Mass Spectrometry, click here. For a list of recharge rates for Peptide Synthesis, click here.
Services
A. Protein Mass Spectrometry and Analysis:
- Two-dimensional gel electrophoresis (available at both Core components)
- Analysis of intact proteins (available at both Core components)
- Proteome mapping (available at both Core components)
- Protein Identification (available at both Core components)
- Protein expression profiling (available at the Proteome Consortium)
- Post-translational modifications (available at both Core components)
- Protein interactions (available at the Proteome Consortium)
- N-terminal sequence analysis (available at the Protein Structure Facility)
- Circular Dichroism Spectroscopy (currently not available)
- Consultation and database searches (available at both Core components)
- Proteome informatics and training (available at the Proteome Consortium)
B. Peptide Synthesis (available at the Protein Structure Facility)
- Custom Peptide Synthesis (including peptides with unusual modifications, MAP and FRET peptides)
- (Multiply) labeled peptides
- Peptide cyclization via lactam formation
- Peptide cyclization via disulfide bond formation
- Peptides
with posttranslational modifications
Equipment:
Sample Preparation, Request Submission and Other Information:
- Proteome Consortium (http://www.proteomeconsortium.org)
- Protein
Structure Core (http://www.med.umich.edu/medschool/brcf/protein/)
Key Personnel
Philip C. Andrews, PhD, Director, Peptide and Proteomics Core and Proteome Consortium
andrewsp@umich.edu; 734 763-3130Henriette Remmer, Ph.D., Co-director, Peptide and Proteomics Core,
hremmer@umich.edu; 734-763-6285
