Major equipment in the Protein Structure Core includes:
Peptide Synthesis
- The Symphony Peptide Synthesizer (PTI Technologies, Inc.) performs fully automated synthesis of 12 peptides simultaneously with synthesis scales ranging from 0.025 mmol to 0.1 mmol
- The 433A Peptide Synthesizer (Applied Biosystems) is a single column peptide synthesizer that operates in "batch" mode. Due to the on-line conductivity reaction monitoring it is an alternative instrument for fully automated synthesis of peptides with difficult sequences at 0.1-1 mmol scales
- Two preparative HPLC instruments (Beckman-Coulter and Varian Inc.) are used for preparative peptide purification of 15-100 mg peptide per injection
- A Beckman Semipreparative HPLC is used for purifications of smaller quantities (2mg-15 mg of peptide)
- A Beckman Analytical HPLC is used for quality control and for determining optimum specifications for the purification of synthetic peptides
- LCQ Duo Ion Trap mass spectrometer with Excalibur software (version
1.2; ThermoFisher).
Protein Mass Spectrometry and Analysis
- UPLC-Qtof premier system for LC-MS/MS analysis (Waters Inc.)
- MALDI micro Mx MALDI-TOF instrument (Waters Inc.)
- IPGphor 3 IEF unit (for 2D gel electrophoresis (GE Healthcare)
- Procise 494HT fully automated N-terminal sequencer (Applied Biosystems/Invitrogen)
- Circular Dichroism Spectrapolarimeter J810 (Jasco Inc.)
- Ultimate capillary HPLC equipped for capillary flow (Dionex)
All instruments listed are maintained by trained facility personnel and
manufacturer's service contracts.
