Morphometric Analysis/MetaMorph
Fluorophore Bleaching:
Original images were collected on an Olympus FV500 confocal microscope. Images were taken pre- and post-bleach of a defined region of interest (red box). Fluorescent signals were measured with MetaMorph software.
Data was plotted with Prism software
Zane Hatahet & Stephen I. Lentz, Ph.D.
Morphology and Image Analysis Core, MDRTC
Neurite Outgrowth:
Original images were collected on a Nikon microscope. Neurite lengths were measured with MetaMorph software.
Data was plotted with Prism software
Katherine Mycek & Joseph M. Corey, M.D. Ph.D.
Department of Neurology
Sections Through Human Sural Nerve
a typical para-phenylinediamine stained section
digital image with the threshold set for dark objects (in this case myelin)
space filling image to analyze fiber size
Axon density was assessed in human sural nerves as an endpoint in drug trials performed at the University of Michigan. Plastic, cross-sections were stained with para-phenylinediamine (PPD) for maximum contrast and digital images used to count myelinated fiber.
Images courtesy of Doug A. Greene, M.D., Kelli Sullivan,
Ph.D. and the MDRTC Nerve Biopsy Core
Micrograph courtesy of John Hayes, JDRF Morphology Core, University of
Michigan
Adipocyte Measurements:
Original images were collected on a Nikon microscope. Image contrast was enhanced with PhotoShop. Adipocyte area and number were measured with MetaMorph software
Data was plotted with Prism software
Tyler C.
Prestwich & Ormond A. MacDougald Ph.D.
Dept. of Molecular and Integrative Physiology
