Skip Navigation

Morphometric Analysis/MetaMorph 

Fluorophore Bleaching:

Fluorophore Bleaching:

Fluorophore Bleach graph

Original images were collected on an Olympus FV500 confocal microscope. Images were taken pre- and post-bleach of a defined region of interest (red box). Fluorescent signals were measured with MetaMorph software.

Data was plotted with Prism software

Zane Hatahet & Stephen I. Lentz, Ph.D.
Morphology and Image Analysis Core, MDRTC

Neurite Outgrowth:

Neurite Outgrowth:

Neurite Outgrowth graph

Original images were collected on a Nikon microscope. Neurite lengths were measured with MetaMorph software.

Data was plotted with Prism software

Katherine Mycek & Joseph M. Corey, M.D. Ph.D.
Department of Neurology

Sections Through Human Sural Nerve

Human Sural

a typical para-phenylinediamine stained section

human sural

digital image with the threshold set for dark objects (in this case myelin)

human sural

space filling image to analyze fiber size

Axon density was assessed in human sural nerves as an endpoint in drug trials performed at the University of Michigan. Plastic, cross-sections were stained with para-phenylinediamine (PPD) for maximum contrast and digital images used to count myelinated fiber.  

Images courtesy of Doug A. Greene, M.D., Kelli Sullivan, Ph.D. and the MDRTC Nerve Biopsy Core
Micrograph courtesy of John Hayes, JDRF Morphology Core, University of Michigan

Adipocyte Measurements:

Adipocyte Measurements:

Original images were collected on a Nikon microscope. Image contrast was enhanced with PhotoShop. Adipocyte area and number were measured with MetaMorph software

Data was plotted with Prism software

graph

Tyler C. Prestwich & Ormond A. MacDougald Ph.D.
Dept. of Molecular and Integrative Physiology