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MIAC Image Gallery

Olympus FV500 LSCM Image Page

Olympus FV500 LSCM Image Page

Colocalization of Zn++ transporter (green) and insulin (red) within insulin secretory granules of INS-1 rat pancreatic cell line

Images were taken on an Olympus FV500 confocal microscope

Courtesy of Antonio R. Lara-Lemus , M.D., Ph.D. and Peter Arvan, M.D., Ph.D., Department of Internal Medicine-Metabolism, Endocrinology & Diabetes, University of Michigan

Confocal Images of Dorsal Root Ganglion Primary Cell Cultures

Confocal Images of Dorsal Root Ganglion Primary Cell Cultures

DRG neurons were stained with a mitochondrial marker (gray, yellow and red) and clumped mitochondria in the soma (yellow) and axons (red) were compared between control and 6 h high glucose

3D z-series images were taken on an Olympus FV500 confocal microscope and puncta were identified with Volocity software

Primary DRG cultures were made in the Laboratory of Eva L. Feldman, M.D., Ph.D., Department of Neurology, University of Michigan

Staining and images were done by Stephen I. Lentz, Ph.D., Laboratory Director of MIAC, University of Michigan

Isolated Primary Dorsal Root Ganglia Neuron In Vitro

Isolated Primary  Dorsal Root Ganglia

Primary Dorsal Root Ganglia cell cultures from embryonic day 15 rats were fixed and stained with fluorescent markers for neurofilament (green), mitochondria (red) and cell nucleus (blue). The 3D Fluorescent image (bottom) was acquired on an Olympus FV500 confocal microscope and then rendered and visualized with Volocity software.

Primary DRG cultures were made in the Laboratory of Eva L. Feldman, M.D., Ph.D., Department of Neurology, University of Michigan

Staining and images were done by Stephen I. Lentz, Ph.D., Laboratory Director of MIAC, University of Michigan

Tissue Sections of Pancreatic Islets from 4 Week Old Control (Wistar) and Diabetic (ZDF) Rats Stained with Antibodies Against Insulin and Nestin

Winstar Insulin westar Insulin Wistar combo

zdf insulin zdf nestin zdf combo

Nestin RBC

overlay Subtract

Images can be processed to subtract auto fluorescence associated with red blood cells (RBC)

From the laboratory of Charles Burant, M.D., Ph.D., Department of Internal Medicine, University of Michigan

Micrographs courtesy of Stephen Lentz, Ph.D., Laboratory Director of MIAC, University of Michigan

Confocal Images Representing Changes in Intracellular Calcium Concentrations Over Time in Neurons and Glia From the Myenteric Plexus

Calcium changes in neurons ( arrow ) loaded with fluo-3 on depolarization with potassium

Calcium changes in glia stimulated by ATP

Micrographs courtesy of Michael W. Mulholland, M.D., Ph.D., Department of Surgery-General , University of Michigan