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FLIP: Fluorescence Loss In Photobleaching

FLIP is the decrease fluorescence in a defined region (red box below)

adjacent to a bleached region (circle below)

FLIP can be used to measure the dynamics of molecular mobility in membranes of living cells

FLIP schematic

 

FLIP Image FLIP graph

Images were collected on the Olympus FluoView 500 laser scanning confocal microscope

MetaMorph software was used to quantify FLIP signal in ROI

Stephen I. Lentz Ph.D. and Stephen A. Ernst Ph.D MDRTC

Edward L. Stuenkel Ph.D. Dept. of Molecular and Integrative Physiology