Research Summary:

The focus of Dr. Weinberg's laboratory is on understanding the cellular pathophysiology of acute renal failure. His current projects are to: 1) Characterize the energetic deficit as it evolves during extended durations of hypoxia/reoxygenation and the effects of protective substrates to ameliorate it under those conditions, 2) Define the mechanisms for the mitochondrial inner membrane abnormalities during the insult and their relative contributions to the energetic deficit, and 3) Assess expression of the lesion and test the efficacy of protective metabolites during ischemia/reperfusion of the kidney in vivo.

Link to PubMed Citations

Selected Publications:

Feldkamp, T., Kribben, A., Roeser, N.F., Senter, R.A., and Weinberg, J. M. Accumulation of nonesterified fatty acids causes the sustained energetic deficit in kidney proximal tubules after hypoxia/reoxygenation. Am. J. Physiol. Renal Physiol. 2005.

Feldkamp, T., Kribben, A., and Weinberg, J. M. F1FO-ATPase activity and ATP dependence of mitochondrial energization in proximal tubules after hypoxia/reoxygenation. J. Am. Soc. Nephrol. 16:1742-1751, 2005.

Feldkamp, T., Kribben, A., and Weinberg, J. M. Assessment of mitochondrial membrane potential in proximal tubules after hypoxia/reoxygenation. Am. J. Physiol. Renal Physiol. 288:F1092-F1102, 2005.

Feldkamp, T., A. Kribben, N.F. Roeser, R.A. Senter, S. Kemner, M.A. Venkatachalam, I. Nissim, and J.M. Weinberg. Preservation of complex I function during hypoxia/reoxygenation-induced mitochondrial injury. Am. J. Physiol. 286:F749-F759, 2004

Weinberg, J.M. and P. Saikumar. Mitochondrial Function. In: M.S. Goligorsky. Methods in Molecular Medicine. Renal Disease: Guide to Technical Approaches. The Humana Press Inc. Totowa , New Jersey . 2003. pp. 351-371.

Zhang, K. J.M. Weinberg, M.A. Venkatachalam, and Z. Dhong. Glycine protection of PC-12 cells against injury by ATP-depletion. Neurochemical Res. 28:893-901, 2003.

Shelden, E.S., J.M. Weinberg, Sorensen, D.R., Edwards, C.A. , and Pollock, F.M. Site-specific alterations of actin assembly visualized in living renal epithelial cells during ATP depletion. J. Am. Soc. Nephrol. 13:2667-2680, 2002.

Weinberg, J.M., M.A. Venkatachalam, and I. Nissim. Pharmacologic and Metabolic Mitochondrial Rescue. In: M. Fink and T. Evans. Mechanisms of Organ Dysfunction in Critical Illness. Springer-Verlag, Berlin. 2001. pp. 69-91.

Weinberg, J.M., M.A. Venkatachalam, N.F. Roeser, R.A. Senter, and I. Nissim. Energetic determinants of tyrosine phosphorylation of proximal tubule focal adhesion proteins during hypoxia/reoxygenation. Am. J. Pathol. 158:2153-2164, 2001.

Dong, Z. , M.A. Venkatachalam, J.M. Weinberg, P. Saikumar, and Y. Patel. Protection of ATP-depleted cells by impermeant strychnine derivatives: Implications for glycine cytoprotection. Am. J. Pathol. 158:1021-1028, 2001.

Dong, Z., M. Denton, J.M. Weinberg, and M.A. Venkatachalam. Death paradigms in cellular hypoxia. In: R. Ruffolo and F. Walsh, Apoptosis in Health and Disease. IPD. Singapore. 2000. pp. 215-242.

Weinberg, J.M., M.A. Venkatachalam, N.F. Roeser, P. Saikumar, Z. Dong, R.A. Senter, and I. Nissim. Anaerobic and aerobic pathways for salvage of proximal tubules from hypoxia-induced mitochondrial injury. Am. J. Physiol. Renal Physiol. 279:F927-F943,2000.

Lin, Z., J.M. Weinberg, R. Malhotra, S. E. Merritt, L. B. Holzman, and F. C. Brosius III. GLUT1 reduces hypoxia-induced apoptosis and JNK pathway activation. Am. J. Physiol. 278:E958-E966, 2000.

Dong, Z., P. Saikumar, Y. Patel, J.M. Weinberg, and M.A. Venkatachalam. Serine protease inhibitors suppress cytochrome c-mediated caspase-9 activation and apoptosis during hypoxia-reoxygenation. Biochem. J. 347:669-677, 2000.

Weinberg, J.M., M.A. Venkatachalam, N.F. Roeser, and I. Nissim. Mitochondrial dysfunction during hypoxia/reoxygenation and its correction by anaerobic metabolism of citric acid cycle intermediates. Proc. Natl. Acad. Sci. U.S.A. 97:2826-2831, 2000.