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Mariam Gonzalez-Hernandez
e-mail address: gonmaria@umich.edu

Mentor: Christiane Wobus, PhD


Research Description

Human Noroviruses (HuNov) in the family Caliciviridae, cause the majority of all non-bacterial gastroenteritis.  Due to the lack of a small animal model, and the inability to culture HuNoV, HuNoV norovirus research is severely hindered.  Since knowledge about the viral life cycle and interactions with its host is currently unknown, we are interested in studying these crucial steps to understand how noroviruses cause disease, and for future development of antiviral therapies by using the only efficient culturable model: murine norovirus (MNV-1). Particularly we are interested in understanding how MNV crosses the epithelial barrier to reach its target cells, macrophages and dendritic cells. My focus is to understand if Microfold (M) cells, which are specialized epithelial cells, are involved in MNV-1 uptake. 


Publications

Cooper, S., Paulsen, M., Ljungman, M., Vu-Phan, D., Kim, D., and Gonzalez-Hernandez, M. 2007. Membrane-elution analysis of content of cyclins A, B1 and E during the unperturbed mammalian cell cycle. Cell Division.2:28

Cooper, S. and Gonzalez-Hernandez, M. 2009. Experimental reconsideration of the utility of serum starvation as a method for synchronizing mammalian cells. Cell Biol. Int.33(1):71-7

Gonzalez-Hernandez, M. B., Bragazzi Cunha, J., Wobus, C.E. 2012. Plaque assay for murine norovirus. J. of Vis. Exp. 66: e4297, doi:10.3791/4297.
 
Gonzalez-Hernandez, M. B., Perry, J.W., Wobus, C.E. 2013. Neutral red assay for murine norovirus replication and detection in a mouse. Bio-protocol.

Elftmann, M. D., Gonzalez-Hernandez, M. B., Kamada, N., Perkins, C. Henderson, K. S., Núñez, G., Wobus, C. E. 2013. Multiple effects of dendritic cell depletion on murine norovirus infection. J. Gen. Virol.



Abstracts

Gonzalez-Hernandez, M.B.; Blanco, L.P.; Auble, H. and Wobus, C.E. 2010. Murine Norovirus is Transcytosed Through a Murine Intestinal Epithelial Cell line in a Sialic Acid- dependent mechanism. Abstract for poster presentation, American Society for Microbiology (ASM), San Diego, CA

Gonzalez-Hernandez, M.B.; Blanco, L.P.; Auble, H., Perry, J.W., Williams, I.R and Wobus, C.E. 2011. Murine norovirus interaction with microfold cells in the intestinal epithelium. Abstract for poster presentation, 10th Annual Immunology Retreat, University of Michigan, Ann Arbor, MI.

Gonzalez-Hernandez, M.B., Perry, J.W., Williams, I.R and Wobus, C.E. 2011. Murine norovirus interaction with the intestinal epithelium. Abstract for oral presentation, American Society for Virology (ASV), University of Minnesota, Minneapolis, MN, July 2011.

Gonzalez-Hernandez, M.B. 2012. Mechanisms of murine norovirus translocation across the intestinal epithelial barrier. Abstract for oral presentation, 11th Annual Immunology Retreat, University of Michigan, Ann Arbor, MI.

Gonzalez-Hernandez, M.B., Liu, T., Blanco, L.P. and Wobus, C.E. 2012. Murine norovirus interaction with an in vitro model of the intestinal epithelium. Abstract for oral presentation, American Society for Virology (ASV), University of Wisconsin, Madison, WI.  (July 21-25, 2012).

Gonzalez-Hernandez, M.B., Yagita, H., Dermody, T. S., Williams, I. R. and Wobus, C.E. 2013. Murine norovirus and reovirus replication in the mouse intestine requires microfold (M) cells. Abstract for poster presentation, Positive Strand RNA Viruses, Boston Park Plaza & Towers, Boston, MA.


Awards

American Society of Microbiology Student Travel Grant, 2010

Rackham Graduate Student Research Grant, 2010


Molecular Mechanisms of Microbial Pathogenesis Training Grant, University of Michigan, 2010-12

Rackham Graduate Student Conference Travel Award, 2011

American Society for Virology Travel Award, 2011

Herman and Dorothy Miller Fund Awardee, 2012

American Society for Virology Travel Award, 2012

Rackham Graduate Student Conference Travel Award, 2013

 

 

 




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