Christiane Wobus, PhD
Human Noroviruses (HuNov) in the family Caliciviridae, cause the majority of all non-bacterial gastroenteritis. Due to the lack of a small animal model, and the inability to culture HuNoV, HuNoV norovirus research is severely hindered. Since knowledge about the viral life cycle and interactions with its host is currently unknown, we are interested in studying these crucial steps to understand how noroviruses cause disease, and for future development of antiviral therapies by using the only efficient culturable model: murine norovirus (MNV-1). Particularly we are interested in understanding how MNV crosses the epithelial barrier to reach its target cells, macrophages and dendritic cells. My focus is to understand if Microfold (M) cells, which are specialized epithelial cells, are involved in MNV-1 uptake.
Cooper, S., Paulsen, M., Ljungman, M., Vu-Phan, D., Kim, D., and Gonzalez-Hernandez, M. 2007. Membrane-elution analysis of content of cyclins A, B1 and E during the unperturbed mammalian cell cycle. Cell Division.2:28
Cooper, S. and Gonzalez-Hernandez, M. 2009. Experimental reconsideration of the utility of serum starvation as a method for synchronizing mammalian cells. Cell Biol. Int.33(1):71-7
Gonzalez-Hernandez, M. B., Bragazzi Cunha, J., Wobus, C.E. 2012. Plaque assay for murine norovirus. J. of Vis. Exp. 66: e4297, doi:10.3791/4297.
Elftmann, M. D., Gonzalez-Hernandez, M. B., Kamada, N., Perkins, C. Henderson, K. S., Núñez, G., Wobus, C. E. 2013. Multiple effects of dendritic cell depletion on murine norovirus infection. J. Gen. Virol.
Gonzalez-Hernandez, M.B.; Blanco, L.P.; Auble, H. and Wobus, C.E. 2010. Murine Norovirus is Transcytosed Through a Murine Intestinal Epithelial Cell line in a Sialic Acid- dependent mechanism. Abstract for poster presentation, American Society for Microbiology (ASM), San Diego, CA
American Society of Microbiology Student Travel Grant, 2010