Zbigniew Zaslona, Ph.D.
e-mail address: zaslona@umich.edu

Mentor: Marc Peters-Golden, M.D.

Techniques Used:

intratracheal administration of agents to mice, isolation of murine primary cells, genotyping of knockout animals, cell culture, flow cytometry and cell sorting, DNA/RNA isolation, real-time PCR, western blots, electrophoretic mobility shift assay, immunohistochemistry, enzyme-linked immunosorbent assay, fluorimetric assays and kinetic analysis of protease activity, gene cloning

Research Description:

Regulation of macrophage alternative regulation by eicosanoids

Publications:

Hecker M, Zasłona Z, Kwapiszewska G, Niess G, Zakrzewicz A, Hergenreider E, Wilhelm J, Marsh LM, Sedding D, Klepetko W, Lohmeyer J, Dimmeler S, Seeger W, Weissmann N, Schermuly RT, Kneidinger N, Eickelberg O, Morty RE. Dysregulation of the Interleukin 13 Receptor System: a Novel Pathomechanism in Pulmonary Arterial Hypertension.
Am J Respir Crit Care Med. 2010 Jun 10. [Epub ahead of print]

Mikulski Z*, Zasłona Z*, Cakarova L, Hartmann P, Wilhelm J, Tecott LH, Lohmeyer J, Kummer W.Serotonin activates murine alveolar macrophages through 5-HT2C receptors. Am J Physiol Lung Cell Mol Physiol. 2010 Aug;299(2):L272-80. Epub 2010 May 21. *both authors contributed equally to the study

Cabanski M, Wilhelm J, Zasłona Z, Steinmüller M, Fink L, Seeger W, Lohmeyer J.Genome-wide transcriptional profiling of mononuclear phagocytes recruited to mouse lungs in response to alveolar challenge with the TLR2 agonist Pam3CSK4. Am J Physiol Lung Cell Mol Physiol. 2009 Oct;297(4):L608-18. Epub 2009 Jul 17.

Zasłona Z, Wilhelm J, Cakarova L, Marsh LM, Seeger W, Lohmeyer J, von Wulffen W. Transcriptome profiling of primary murine monocytes, lung macrophages and lung dendritic cells reveals a distinct expression of genes involved in cell trafficking. Respir Res. 2009 Jan 16;10:2.

Awards:

World Immune Regulation Meeting III, Davos, Switzerland, 22 - 25 March 2009. Best Poster Prize ”Late breaking abstracts”