JiHoon Chang, Ph.D.
e-mail address: jihoonc@umich.edu

Mentor: Cheong-Hee Chang, Ph.D.

Techniques Used:

ELISA, qRT-PCR, immunoblottings et cetera


Research Description:

Upon infection with virus, bacteria or parasites, it is essential for the host to mount a rapid inflammatory response to invading pathogens. The pro-inflammatory response is crucial for fighting and clearing the pathogens. , However, on occasion, excessive or uncontrolled inflammation has detrimental effects for the host and results in inflammatory diseases such as rheumatoid arthritis, inflammatory bowel disease and septic shock. Dendritic cells (DC) play a key role in keeping the immune response in a balance. Upon infection, DC can produce many first-line defensive cytokines including IL-1, IL-6, IL-12, and tumor necrosis factor- a (TNF- a) . Cytokines produced by DC can shape the adaptive immune response in many other immune cells. DC can also produce anti-inflammatory cytokines such as IL-10 to limit and terminate the inflammatory responses.

I'm interested in the immune regulation by DC. The focus of my work in the Chang lab has been the negative regulation of cytokine, especially IL-10 and IL-23, at the molecular level.

Publications:

JiHoon Chang, and Cheong-Hee Chang. 2009. Negative regulation of MyD88-dependent signaling by interleukin-10 in dendritic cells. in preparation.

JiHoon Chang, Sebenzile K. Myeni , Tsang Long Lin , Ching Ching Wu , Chris J. Staiger and Daoguo Zhou . 2007. SipC multimerization promotes actin nucleation and contributes to Salmonella -induced inflammation . Molecular Microbiology 66(6):1548-1556.

JiHoon Chang, Jue Chen and Daoguo Zhou. 2005. Delineation and characterization of the actin nucleation and effector translocation activities of Salmonella SipC. Molecular Microbiology 55(5):1379-1389.

JangSeong Kim, JiHoon Chang, HyunKyung Yu, JinHyung Ahn, JungSun Yum, SukKeun Lee, KyungHwan Jung, DooHong Park, Yeup Yoon, SiMyung Byun and SooIl Chung. 2003. Inhibition of angiogenesis and angiogenesis-dependent tumor growth by the cryptic kringle fragments of human apolipoprotein(a). Journal of Biological Chemistry 278(31):29000-29008.

JangSeong Kim, JiHoon Chang, SooIl Chung and JungSun Yum. 2001. Importance of the host genetic background on immune responses to Helicobacter pylori infection and therapeutic vaccine efficacy. FEMS Immunology and Medical Microbiology 31(1):41-46.

JangSeong Kim, JiHoon Chang, WonYoung Seo, GumJu Yu, SooIl Chung and JungSun Yum. 2000. Cloning and characterization of a 22 kDa outer-membrane protein (Omp22) from Helicobacter pylori . Molecules and Cells 10(6):632-640.

EunJeong Park, JiHoon Chang, JangSeong Kim, JungSun Yum and SooIl Chung. 2000. The mucosal adjuvanticity of two nontoxic mutants of Escherichia coli heat-labile enterotoxin varies with immunization routes. Experimental and Molecular Medicine 32(2):72-78.

JangSeong Kim, JiHoon Chang, SooIl Chung and JungSun Yum. 1999. Molecular cloning and characterization of the Helicobacter pylori fliD Gene, an essential factor in flagellar structure and motility. Journal of Bacteriology 181(22):6969-6976.

Eun Jeong Park, JiHoon Chang, JangSeong Kim, SooIl Chung and JungSun Yum. 1999. Development of two novel nontoxic mutants of Escherichia coli heat-labile enterotoxin. Experimental and Molecular Medicine 31(2):101-107.

JiHun Chang, SoonSang Yoon, SangMoon Lee, Iha Park, DoYoung Jung, YoungSik Park and JeongBin Yim. 1999. Purification and properties of novel calcium-binding proteins from Streptomyces coelicolor . The Journal of Microbiology 37(1):21-26.

Honors and Awards: