Ende Zhao, M.D.
e-mail address: endezhao@umich.edu

Mentor: Weiping Zou, M.D.,Ph.D.

Techniques Used:

FACS, IHC, IF, ELISA, Elis-pot, ChIP, Thymidine incorporation, qRT-PCR, Western blotting, Plasmid DNA construction, digestion and ligation, Lentivirus packaging, Transfection and transduction, Electrophoresis, Mouse models, BMD and BMC measurement, in vivo bioluminescent imaging and radiographic imaging.

Research Description:

Bone marrow is thought to be a primary hematopoietic organ. However, accumulated evidences demonstrate that active function and trafficking of immune cells including Treg cells are observed in the bone marrow. Furthermore, bone marrow is a predetermined metastatic location for multiple human tumors including prostate cancer. And Treg cells contribute to self-tolerance and tumor immune pathology. Our research is designed to investigate the role of Treg cells in patients with prostate cancer and the phenotype and function of human T cells.

Publications:

Zhao E, Xu H, Wang L, Kryczek I, Wu K, Hu Y, Wang G, Zou W. Bone marrow and the control of immunity. Cell Mol Immunol. advance online publication 2011 Oct. 24.

Kryczek I, Zhao E, Liu Y, Wang Y, Vatan L, Szeliga W, Moyer J, Klimczak A, Lange A, Zou W. Human TH17 Cells Are Long-Lived Effector Memory Cells. Sci. Transl. Med. 2011 Oct 12;3(104):104ra100.

Wilke CM, Kryczek I, Wei S, Zhao E, Wu K, Wang G, Zou W. Th17 cells in cancer: help or hindrance? Carcinogenesis. 2011 May;32(5):643-9.

Kryczek I, Wu K, Zhao E, Wei S, Vatan L, Szeliga W, Huang E, Greenson J, Chang A, Rolinski J, Radwan P, Fang J, Wang G, Zou W. IL-17+ Regulatory T Cells in the Microenvironments of Chronic Inflammation and Cancer. J Immunol. 2011 Apr 1;186(7):4388-95.

Wilke CM, Wu K, Zhao E, Wang G, Zou W. Prognostic significance of regulatory T cells in tumor. Int J Cancer. 2010 Aug 15;127(4):748-58.

Honors and Awards: