Service Estimates
Estimates for Frequently Used Hybridoma Core Services
Full costs (Discounts for RDCC or MDRTC members are available)
Monoclonal Antibody Development Project
Screening of sera and culture supernatants by investigator’s lab.
Costs will increase as more clones are expanded, cryopreserved, subcloned and etc.
Service |
Rate |
Total |
Immunize, 3 BALB/c mice (1 i) |
$725.00 |
$725.00 |
Fusion, 1 spleen, 480 X 96 wells (1 f2) |
$770.00 |
$770.00 |
Expand 20 primary cultures for rescreening (20 tc1) |
$28.00 |
$560.00 |
Subclone 4 primary cultures (4 c) |
$110.00 |
$440.00 |
Expand 10 primary cultures for freezing and storage (10 tc2) |
$32.00 |
$320.00 |
Freeze two vials per hybridoma primary culture, includes 3 years storage (10 cryo) |
$19.50 |
$190.50 |
Expand 2 clones from each of 3 primary cultures to 24 well plate culture (6 tc1) |
$28.00 |
$168.00 |
Expand 6 hybridoma clones to 6 well plate cultures (6tc2) |
$32.00 |
$192.00 |
Freeze two vials per hybridoma subclone (6 cryo) |
$19.50 |
$117.00 |
Grow cells from three hybridomas for ascites productions (6tc3) |
$24.00 |
$144.00 |
in vivo antibody production, 3 hybridomas, 2 mice per cell line (3ap1) |
$328.00 |
$984.00 |
isotype determination, 3 hybridomas (3iso) |
$38.50 |
$115.50 |
Estimated Total Cost |
|
$4,726.00 |
Thaw and Cryopreserve a Hybridoma Cell Line
Approximately 50 ml of culture supernatant can be saved from this work and provided to lab if requested in advance.
Service |
Rate |
Total |
Thaw 1 vial cells (1 ct) |
$25.00 |
$25.00 |
Expand culture (1 tc2) |
$32.00 |
$32.00 |
Grow cells for cryopreservation (5 tc3) |
$24.00 |
$120.00 |
Freeze cells (3 cryo = 6 vials, includes storage in our facility for 3 years) |
$19.50 |
$58.50 |
Estimated Total Cost |
|
$235.50 |
Monoclonal Antibody Production in vitro in CL-1000 high density culture
Does not include thawing or cryopreserving cells. Harvest antibody concentrations typically range from 0.25 - 2 mg/ml.
Service |
Rate |
Total |
Grow cells to innoculate flask (6 tc4) |
$17.00 |
$102.00 |
set up flask, culture 30-45 days, collect 4-5 harvests @ approx. 10 ml ea (1 ap5) |
$846.00 |
$846.00 |
Estimated Total Cost |
|
$948.00 |
Monoclonal Antibody Production as Ascites in 2 BALB/c Mice
Does not include thawing or cryopreserving cells. Antibody concentration typically ranges from 1 - 10 mg/ml.
Service |
Rate |
Total |
Grow cells to inject mice (3 tc4) |
$17.00 |
$51.00 |
in vivo antibody production, 1 hybridoma, 2 Balb/c mice (1 ap1) |
$328.00 |
$328.00 |
Estimated Total Cost |
|
$379.00 |
