BIOARTIFICIAL RENAL EPITHELIAL CELL SYSTEM (BRECS)

Background
Renal cell therapy has shown clinical efficacy in the treatment of acute renal failure (ARF), and promise for treatment of end stage renal disease (ESRD) by supplementing conventional small solute clearance (hemodialysis or hemofiltration) with endocrine and metabolic function provided by cells maintained in an extracorporeal circuit.

The problem
A major obstacle to the widespread adoption of cell therapy is the lack of a system to distribute and store cell therapy devices for use at point of care facilities.

Our solution:
Our lab has designed the first all-in-one culture vessel, cryostorage device, and cell therapy delivery system. Our BRECS device was loaded with up to 20 cell-seeded porous disks, which were maintained by perfusion culture. Once cells reached over 5 x 10
6 cells/disk for a total therapeutic dose of approximately10 8 cells, the BRECS was cryopreserved for storage at -80 or -140°C. The BRECS was rapidly thawed and perfusion culture was resumed. Near pre-cryopreservation values of cell viability, metabolic activity and differentiated phenotype of functional renal cells were confirmed post-reconstitution.

The results are described in Cell Medicine, Volume 4, Number 1, 2012 , pp. 33-43(11:

Bioartificial Renal Epithelial Cell System (BRECS): A Compact, Cryopreservable Extracorporeal Renal Replacement Device

The promise...
This technology could be extended to administer other cell-based therapies where metabolic, regulatory or secretion functions can be leveraged in an immuno-isolated extracorporeal circuit.

BRECS
The world's first all-in-one culture vessel, cryostorage device and cell therapy delivery system.
 
 
   

References for the Bioartificial Renal Epithelial Cell System (BRECS):

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