Training
Flow Cytometry

No training is required to bring samples to the facility to be run by our trained technicians. However, we do offer training on all core instrumentation for those users wanting to take advantage of the discounted rate for self-operation or for those wanting to utilize the equipment during the off-hours.

The training consists of two steps. The first is a mandatory lecture. This lecture is held monthly, and a place can be reserved by contacting the core staff.

Following the initial lecture, analyzer users will be allowed to schedule for a two-hour "hands on" training session on the analyzer of their choice. Additional training time can be scheduled if required. Those requesting cell sorter training must first demonstrate a marked aptitude in flow cytometry and analyzer operation.

Instructor: David J Adams
Phone: 734.647.3216
University of Michigan Flow Core Web Site
Email: davadams@umich.edu  (Any Time)
Office: M-F 9AM to 5PM EST

An Introduction to Flow Cytometry

Greetings and Welcome to University of Michigan Flow Cytometry Core Facility. My name is David Adams, and I am the Managing Director for the Flow Core. I've been working in and training people in flow cytometric techniques since 1992, and I am always excited by the opportunity to introduce investigators to this powerful technique.

If you are interested in learning about flow cytometry, I have prepared a brief tutorial which is presented below. Each section and associated exercise should take no longer than one hour to complete. If you have questions at any time, feel free to stop by the flow lab and speak to me directly. I am also readily available by phone or email throughout the day.

Course Outline

Module One - Introduction to Flow Cytometry

This module covers basic flow cytometry theory by reviewing the following topics:

Module Two - University of Michigan FACSCalibur Calibration and Operation

This module is designed to cover the very basics of FACSCalibur operation. This module will cover:

Requirements

There is no required text for this course. If you are interested in developing a library of cytometry-related reference materials, my recommendations can be found here.

All video presentations on this site are in QuickTime Movie format. If you do not have QuickTime currently installed, you may find it here.

Adobe Acrobat Reader will also be required to view some of the course resources. If you do not have Acrobat Reader currently installed, you may find it here.

Again, please feel free to contact me if you have any questions whatsoever regarding this course or core facility resources.

Dave Adams