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University of Michigan

Flow Cytometry Core Facility

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Protocols on Demand

The following protocols are available for download (pdf):

  1. General Surface Antibody Staining Procedure
  2. Cell Proliferation by Flow Cytometry (BrdU and PI) (Sample Data)
  3. Cell Proliferation by Flow Cytometry (Acridine Orange)
  4. Propidium Iodide DNA Labeling following Ethanol Fixation
  5. Propidium Iodide DNA Labeling for Sub-G1 Analysis: Hypotonic Lysis
  6. Hoechst 33342 DNA Staining (Viable Cell Cycle Analysis)
  7. Calcium Flux via Indo-1

Please stop by the main lab or call if you have questions. Procedure authors are indicated as appropriate.


Last updated November 15, 2007 dja