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Lab Menu
The laboratory
physical plant consists of 900 square feet of space for bench and
in vivo experimentation. Equipment is in place for the conduct of
in vivo and in vitro experiments, the use of radioisotopes,
and lung infection by human pathogens including Pseudomonas aeruginosa
and Klebsiella pneumoniae. Techniques currently in use (all
either previously published or currently being performed) include:
In vivo
Lung Injury Systems
- Hemorrhagic
shock
- Intravascular
complement activation
- Intrapulmonary
immune complex formation
- Bacterial
pneumonia
Ex vivo
Lung Injury Systems
- Isolated
perfused lung apparatus
- Allows
determination of arterial, venous, and capillary resistances,
pre- and post-capillary resistance, and pulmonary vascular
filtration coefficients
Lung Injury
Measurements
- Lung and
BAL neutrophil quantification
- Endothelial
barrier function
- Including
vascular single- and dual radioisotope methods and extravascular
lung water determinations
- Light and
transmission electron microscopy
- Static lung
compliance
- Gas exchange
Microbiological
Techniques
- Quantitative
culture of blood and organs
- Bacterial
serotyping by antigenic and genetic analysis
- Allele replacement
for targeted mutation of bacteria
- Hyperimmune
serum production from heat killed bacteria
Complement
Techniques
- Classical,
alternative, and MBL CH50 for human, rat, and mouse
- Quantitative
determination of C3 opsonization on bacterial surfaces (human
and mouse) using flow cytometry
- Activation
pathway identification on bacterial surfaces using selective divalent
cation chelation and competitive saccharide techniques
- Murine C5
convertase activity using immunoblot
- Identification
of C5 convertase catalytic products using MALDI/TOF (on line Autumn
2002)
- Purification
of cobra venom factor via LPLC
- Production
of in vivo complement depletion using CVF
- Access to
C3-/- and C5-/- mouse lines
Cell Signaling
Techniques
- Analysis
of FcgIIR signaling pathway, particularly phosphorylation of syk
Analysis
of Gene Expression
- RNAase protection
assays (32P method)
- Murine cDNA
microarray analysis (on line, 2002-2003)
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