Bioluminescence

IVIS Spectrum View

PerkinElmer's IVIS™ Spectrum imaging system The IVIS® Spectrum in vivo imaging system uses a novel patented optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. The IVIS Spectrum is a versatile and advanced in vivo imaging system. An optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. The system includes a highly sensitive camera, a dark imaging chamber to minimize incident light, and software to quantify the results. The IVIS imaging systems start with a back-thinned CCD camera, designed for high-efficiency photon detection, particularly in the important red region of the spectrum. It can detect very small numbers of photons as well as operate as a traditional camera; displaying images in that wide signal range is a function of the system's Living Image™ software. The CCD is cooled and the electronic readout is optimized so that the data gathered to create the real-time in vivo images have extremely low noise. Up to 5 animals can be imaged at a time and thermoregulation is achieved by means of a heated stage. Inhalable anesthesia is delivered via nose cones throughout the imaging procedure.


Fluorescence

IVIS Spectrum View

PerkinElmer's IVIS™ Spectrum imaging system The IVIS® Spectrum in vivo imaging system uses a novel patented optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. The IVIS Spectrum is a versatile and advanced in vivo imaging system. An optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. The system includes a highly sensitive camera, a dark imaging chamber to minimize incident light, and software to quantify the results. The IVIS imaging systems start with a back-thinned CCD camera, designed for high-efficiency photon detection, particularly in the important red region of the spectrum. It can detect very small numbers of photons as well as operate as a traditional camera; displaying images in that wide signal range is a function of the system's Living Image™ software. The CCD is cooled and the electronic readout is optimized so that the data gathered to create the real-time in vivo images have extremely low noise. Up to 5 animals can be imaged at a time and thermoregulation is achieved by means of a heated stage. Inhalable anesthesia is delivered via nose cones throughout the imaging procedure. For advanced fluorescence pre-clinical imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images. The instrument is equipped with 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal.

MR

Agilent 4.7T View

The Center for Molecular Imaging is a leading facility for magnetic resonance imaging (MRI) of animal models. Medium-to-large animal MR experiments are performed on a state-of-the-art Agilent (formerly Varian) 4.7 Tesla horizontal bore system. Physiological monitoring is available and animals may be anesthetized using injectable drugs or with a reliable gas delivery system. Thermoregulation is accomplished with reusable isothermal pads. A variety of volume coils are available, accommodating samples of many sizes and geometries.