Kathleen Collins, M.D., Ph.D.
Assistant Professor of Internal Medicine
Assistant Professor of Microbiology and Immunology

Brian Athey, Ph.D.
Assistant Professor of Cell and Developmental Biology
Director, Michigan Ctr. for Biological Information (OVPR)
Research Investigator, Ctr. for Biologic Nanotechnology

Marc Lippman, M.D.
John G. Searle Professor of Internal Medicine
Chair, Department of Internal Medicine

Michael Imperiale, Ph.D.
Professor of Microbiology and Immunology
 

Michael Peacock

Laboratory Supervisor, UM Vector Core Laboratory
 

Stephen Johnston, Ph.D.
Professor of Internal Medicine and Biochemistry
Director, Center for Biomedical Inventions
University of Texas Southwestern

Russell, S.J., S.H. Reed, W. Huang, E.C Friedberg and S.A. Johnston. (1999) The 19S regulatory complex of the proteasome functions
independently of proteolysis in nucleotide excision repair. Mol.Cell 3: 687-695.

Russell, S.J., K.A. Steger and S.A. Johnston (1999) Sub-cellular localization, stochiometry and protein levels of 26S proteasome
subunits in yeast. J. Biol. Chem. 274(31):21943-52.

Sykes, K.A. and S.A. Johnston. (1999) Genetic-live vaccines mimic the antigenicity but not pathogenicity of live viruses.
DNA, Cell Biology 18(7):521-31

O’Farrell, P., F. Gonzalez, W. Zheng, S.A. Johnston and L. Joshua-Tor (1999) Crystal structure of human bleomycin hydrolase, a
self-compartmentalizing cysteine protease Structure 7: 619-27.

Sykes, K. and S.A. Johnston (1999) Linear Expression Elements: a rapid, in vivo method to screen for gene functions.
Nature-Biotech 17: 355-359.

Timares, L., A. Takashima and S.A. Johnston (1998) Quantitative analysis of the immunopotentcy of genetically transfected
dendritic cells. Proc.Nat.Acad.Sci. 22: 13147-13152.

Zheng, W. and S.A. Johnston (1998) The nucleic acid binding activity of bleomycin hydrolase is involved in bleomycin detoxification.
Mol. Cell Biol. 18: 3580-3585.

Corton, J.C., E. Moreno and S.A. Johnston (1998) Alterations in the Gal4 DNA binding domain can affect transcriptional
activation independent of DNA binding. J. Biol. Chem.273: 13776-13780

Zheng, W., S. A. Johnston and L. Joshua-Tor (1998) The unusual active site of Gal6/bleomycin hydrolase can act as a carboxypeptidase,
aminopeptidase and peptide ligase. Cell 93: 103-110

Alnawaz Rehemtulla, Ph.D.

Associate Professor of Radiation Oncology

Fridman JS, Parsels J, Rehemtulla A, Maybaum J., Cytochrome c depletion upon expression of Bcl-XS.
J Biol Chem. 2001 Feb 9;276(6):4205-10.

Hamstra DA, Rice DJ, Pu A, Oyedijo D, Ross BD, Rehemtulla A., Combined radiation and enzyme/prodrug treatment
for head and neck cancer in an orthotopic animal model. Radiat Res. 1999 Nov;152(5):499-507.

Donna Shewach, M.D.

Professor of Pharmacology

Robinson BW, Shewach DS. Radiosensitization by gemcitabine in p53 wild-type and mutant MCF-7 breast
carcinoma cell lines. Clin Cancer Res. 2001 Aug;7(8):2581-9.

Eisbruch A, Shewach DS, Bradford CR, Littles JF, Teknos TN, Chepeha DB,
Marentette LJ, Terrell JE, Hogikyan ND, Dawson LA, Urba S, Wolf GT, Lawrence TS. Radiation concurrent with gemcitabine for 
locally advanced head and neck cancer:
a phase I trial and intracellular drug incorporation study. J Clin Oncol. 2001 Feb 1;19(3):792-9.

Morishima Y, Kanelakis KC, Murphy PJ, Shewach DS, Pratt WB. Evidence for iterative ratcheting of receptor-bound hsp70 between 
its ATP and ADP conformations during assembly of glucocorticoid receptor.hsp90 heterocomplexes. 
Biochemistry. 2001 Jan 30;40(4):1109-16.

Parker WB, Shaddix SC, Rose LM, Waud WR, Shewach DS, Tiwari KN, Secrist JA
3rd. Metabolism of 4'-thio-beta-D-arabinofuranosylcytosine in CEM cells. Biochem Pharmacol. 2000 Dec 15;60(12):1925-32.

Ostruszka LJ, Shewach DS. The role of cell cycle progression in radiosensitization by 2',2'-difluoro-2'-deoxycytidine. 
Cancer Res. 2000 Nov 1;60(21):6080-8.

Boucher PD, Ostruszka LJ, Shewach DS. Synergistic enhancement of herpes simplex virus 
thymidine kinase/ganciclovir-mediated cytoxicity by hydroxyurea. Cancer Res. 2000 Mar 15;60(6):1631-6.

Lawrence TS, Eisbruch A, McGinn CJ, Fields MT, Shewach DS. Radiosensitization by gemcitabine. 
Oncology (Huntingt). 1999 Oct;13(10 Suppl 5):55-60. Review.

Parker WB, Shaddix SC, Rose LM, Shewach DS, Hertel LW, Secrist JA 3rd,
Montgomery JA, Bennett LL Jr. Comparison of the mechanism of cytotoxicity of 2-chloro-9-(2-deoxy-2-
fluoro-beta-D-arabinofuranosyl)adenine, 2-chloro-9-(2-deoxy-2-fluoro- beta-D-ribofuranosyl)adenine, and 
2-chloro-9-(2-deoxy-2,2-difluoro-beta-D-ribofuranosyl)adenine in CEM cells. Mol Pharmacol. 1999 Mar;55(3):515-20.

Rubsam LZ, Boucher PD, Murphy PJ, KuKuruga M, Shewach DS. Cytotoxicity and accumulation of ganciclovir triphosphate 
in bystander cells cocultured with herpes simplex virus type 1 thymidine kinase-expressing human
glioblastoma cells. Cancer Res. 1999 Feb 1;59(3):669-75.

Rubsam LZ, Davidson BL, Shewach DS. Superior cytotoxicity with ganciclovir compared with acyclovir and
1-beta-D-arabinofuranosylthymine in herpes simplex virus-thymidine
kinase-expressing cells: a novel paradigm for cell killing.
Cancer Res. 1998 Sep 1;58(17):3873-82.

Svend Freytag, Ph.D.

Research Investigator

Henry Ford Health System

Heilig CW, Kreisberg JI, Freytag S, Murakami T, Ebina Y, Guo L, Heilig K,
Loberg R, Qu X, Jin Y, Henry D, Brosius FC 3rd. Antisense GLUT-1 protects mesangial cells from glucose induction of 
GLUT-1 and fibronectin expression. Am J Physiol Renal Physiol. 2001 Apr;280(4):F657-66.

Rogulski KR, Freytag SO, Zhang K, Gilbert JD, Paielli DL, Kim JH, Heise CC, Kirn DH.
In vivo antitumor activity of ONYX-015 is influenced by p53 status and is augmented by radiotherapy.
Cancer Res. 2000 Mar 1;60(5):1193-6.

Rogulski KR, Wing MS, Paielli DL, Gilbert JD, Kim JH, Freytag SO. Double suicide gene therapy augments the 
antitumor activity of a replication-competent lytic adenovirus through enhanced cytotoxicity and radiosensitization.
Hum Gene Ther. 2000 Jan 1;11(1):67-76.

Xie Y, Gilbert JD, Kim JH, Freytag SO. Efficacy of adenovirus-mediated CD/5-FC and HSV-1 thymidine 
kinase/ganciclovir suicide gene therapies concomitant with p53 gene therapy.
Clin Cancer Res. 1999 Dec;5(12):4224-32.

Kim JH, Kolozsvary A, Rogulski K, Khil MS, Brown SL, Freytag SO. Selective radiosensitization of 9L glioma i
n the brain transduced with doublesuicide fusion gene. Cancer J Sci Am. 1998 Nov-Dec;4(6):364-9.

Gabel M, Kim JH, Kolozsvary A, Khil M, Freytag S. Selective in vivo radiosensitization by 5-fluorocytosine of 
human colorectal carcinoma cells transduced with the E. coli cytosine deaminase (CD) gene.
Int J Radiat Oncol Biol Phys. 1998 Jul 1;41(4):883-7.

Adelmant G, Gilbert JD, Freytag SO. Human translocation liposarcoma-CCAAT/enhancer binding protein (C/EBP)
homologous protein (TLS-CHOP) oncoprotein prevents adipocyte differentiation by directly interfering with C/EBPbeta function.
J Biol Chem. 1998 Jun 19;273(25):15574-81.

Freytag SO, Rogulski KR, Paielli DL, Gilbert JD, Kim JH. A novel three-pronged approach to kill cancer cells selectively: 
concomitant viral, double suicide gene, and radiotherapy. Hum Gene Ther. 1998 Jun 10;9(9):1323-33.

Michael Clarke, M.D.

Professor of Internal Medicine

Weber, B.L., Westin, E. and Clarke, M.F.: Differentiation of mouse erythroleukemia cells enhanced by alternatively spliced c-myb mRNA. Science, 249:1291-1293, 1990.

Ryan, J.J., Danish, R., Gottlieb, C.A. and Clarke, M.F.: Cell cycle analysis of p53 induced cell death in murine erythroleukemia cells. Mol & Cell Biol, 13:711-719, 1993.

Ryan, J.J., Prochownik, E.V., Gottlieb, C.A., Apel, I., Merino, R., Nunez, G. and Clarke, M.F.: c-myc and bcl-2 modulate p53 function by altering p53 subcellular trafficking during the cell cycle. PNAS, 91:5878-5882, 1994.

Clarke, M.F., Apel, I.A., Eipers, P.G., Sumantran, V., Gonzalez-Garcia, M., Benedict, M., Pipaya, G., Fukunaga, N., Davidson, B., Long, M., Minn, A.J., Boise, L.H., Thompson, C.B., Wicha, M. and Nunez, G.: A recombinant bcl-xs adenovirus selectively induces apoptosis in cancer cells, but not normal bone marrow cells. PNAS, 92:11024-11028, 1995.

Liang, S.-H., Hong, D. and Clarke, M.F.: Cooperation of a single lysine mutation and a C-terminal domain in the cytoplasmic sequestration of the p53 protein. J Biol Chem, 273:19817-19821, 1998.

Han, J.S., Qian, D., Wicha, M.S. and Clarke, M.F.: A method of limited replication for the efficient in vivo delivery of adenovirus to cancer cells. Human Gene Therapy, 9:1209-1216, 1998.

Liang, S.-H. and Clarke, M.F.: The nuclear import of p53 is determined by the presence of a basic domain and its relative position to the nuclear localization signal. Oncogene, 18 (12), 2163-2166, 1999.

Gregory M. Christman, M.D.

Director of Assisted Reproductive Technology

Assistant Professor of Obstetrics and Gynecology

Lab homepage

Christman GM, McCarthy JD.: Gene therapy and uterine leiomyomas. Clin Obstet Gynecol. 2001 Jun;44(2):425-35. Review.

Padmanabhan V, Christman GM, Randolph JF, Kelch RP, Marshall JC, Beitins IZ. Dynamics of bioactive follicle-stimulating hormone secretion in women with polycystic ovary syndrome: effects of estradiol and progesterone. Fertil Steril. 2001 May;75(5):881-8.

Menge AC, Christman GM, Ohl DA, Naz RK. Fertilization antigen-1 removes antisperm autoantibodies from spermatozoa of
infertile men and results in increased rates of acrosome reaction. Fertil Steril. 1999 Feb;71(2):256-60.

Beilin Y, Bodian CA, Mukherjee T, Andres LA, Vincent RD Jr, Hock DL, Sparks AE, Munson AK, Minnich ME, Steinkampf MP, Christman GM, McKay RS, Eisenkraft JB. The use of propofol, nitrous oxide, or isoflurane does not affect the
reproductive success rate following gamete intrafallopian transfer (GIFT): a multicenter pilot trial/survey. Anesthesiology. 1999 Jan;90(1):36-41.

Smith YR, Randolph JF Jr, Christman GM, Ansbacher R, Howe DM, Hurd WW. Comparison of low-technology and high-technology monitoring of clomiphene citrate ovulation induction. Fertil Steril. 1998 Jul;70(1):165-8.

Niu H, Simari RD, Zimmermann EM, Christman GM. Nonviral vector-mediated thymidine kinase gene transfer and ganciclovir
treatment in leiomyoma cells. Obstet Gynecol. 1998 May;91(5 Pt 1):735-40.

Zimmermann EM, Li L, Hou YT, Cannon M, Christman GM, Bitar KN. IGF-I induces collagen and IGFBP-5 mRNA in rat intestinal smooth muscle. Am J Physiol. 1997 Oct;273(4 Pt 1):G875-82.

Bergman CA, Talavera F, Christman GM, Baker VV, Roberts JA, Menon KM. Transforming growth factor-beta negatively modulates proliferation and c-fos expression of the human endometrial adenocarcinoma cell line HEC-1-A. Gynecol Oncol. 1997 Apr;65(1):63-8.

Hurd WW, Randolph JF Jr, Christman GM, Ansbacher R, Menge AC, Gell JS. Luteal support with both estradiol and progesterone after clomiphene citrate stimulation for in vitro fertilization. Fertil Steril. 1996 Oct;66(4):587-92.

John V. Moran, Ph.D.

Assistant Professor of Human Genetics

Lab Homepage

Moran JV DeBernardinis RJ and Kazazian HH: Exon Shuffling by LI Retrotransposition. Science 283, 1530-34, 1999.

Kazazian HH Jr and Moran JV: The impact of L1 retrotransposons on the human genome. Nature Genetics 19, 19-24, 1998.

Naas TN, DeBerardinis RJ, Moran JV, Kingsmore SF, Seldin MF, Hayashizaki Y, Martin SL and Kazazian HH: An actively-retrotransposing novel subfamily of mouse L1elements. EMBO Journal 17, 590-97, 1998.

Sassaman DM, Dombroski BA, Moran JV, Kimberland ML, Naas TP, DeBerardinisRJ, Gabriel A, Swergold GD, and Kazazian HH : Many human L1 elements are capable of retrotransposition. Nature Genetics 16, 37-43, 1997.

Moran JV, Holmes SE, Naas TP, DeBerardinis RJ, Boeke JD and Kazazian HH: High frequency retrotransposition in cultured mammalian cells. Cell 87, 917-27, 1996.

Feng Q, Moran JV, Kazazian HH and Boeke JD : Human L1 encodes a conserved endonuclease required for retrotransposition. Cell 87, 905-16, 1996.

Moran JV, Zimmerly SA, Eskes R, Kennell JC, Butow RA, Lambowitz AM and Perlman PS: Mobile group II introns of yeast mitochondrial DNA are novel site-specific retroelements. Molecular and Cellular Biology 15, 2828-2838, 1995.

Moran JV, Mecklenburg KL, Sass P., Belcher SM, Mahnke D, Lewin A and Perlman, PS: Splicing defective mutants of the COX1 gene of yeast mitochondrial DNA: initial definition of the maturase domain of the group II intron aI2. Nucleic Acids Research 11, 2057-64, 1994.

Kennell JC, Moran JV, Perlman PS, Butow RA and Lambowitz AM : Reverse transcriptase activity associated with maturase-encoding group II introns in yeast mitochondria. Cell 73, 133-46, 1993.

Moran JV, Wernette CM, Mecklenburg KL, Butow RA and Perlman PS: Intron 5a of the COX1 gene of
yeast mitochondrial DNA is a mobile group I intron. Nucleic Acids Research 20,4069-76, 1992.