The research in Pamela Raymond's laboratory is concerned
with retinal neurogenesis, neural stem cells, and regeneration.
The overall objective is to understand the molecular basis of cell-cell
interactions that regulate retinal neurogenesis (the formation of
neurons) and neuronal specificity (the expression of differentiated
cellular features) during development and regeneration. We use zebrafish
as a model system, and we concentrate on a selected subset of molecular
interactions that are likely to be involved in determination of
cell fate in retinal stem cells and progenitor cells: cell surface
signaling molecules - such as the cadherin family of adhesion molecules,
the Notch-Delta signaling pathway, and other signaling systems,
and transcriptional regulators - especially the family of paired-like
homeodomain proteins that have been implicated in the regionalization
of brain territories, the control of proliferation, and the determination
of specified progenitor cells and differentiated cell fates. We
are also interested in understanding the cellular and molecular
mechanisms of neuronal pattern formation, especially the formation
of precise arrays of cone photoreceptors in the teleost fish retina.
Representative Publications:
- Braisted, J.E., T.F. Essman and P.A. Raymond. 1994. Selective regeneration of photoreceptors in goldfish retina. Development 120:2409-2419.
- Stenkamp, D.L., R.A. Frey, S.N. Pradhudesai and P.A. Raymond. 2000. Function for hedgehog genes in zebrafish retinal development. Devel. Biol. 220:238-252.
- Chuang, J.C. and P.A. Raymond. 2001. Zebrafish genes rx1 and rx2 help define the forebrain region that gives rise to retina. Devel. Biol. 231:13-30.
- Chuang, J.C. and P.A. Raymond. 2002. Embryonic origin of the vertebrate retina: new perspectives from studies on zebrafish. BioEssays 24:1-11.
- Shen, Y.-C. and P.A. Raymond. 2004. Zebrafish cone-rod (crx) homeobox gene promotes retinogenesis. Devel. Biol. 269:237-251.
- Raymond, P.A. 2004. Restoration of vision. In Handbook of Stem Cells: Volume 2: Adult & Fetal Stem Cells, R. Lanza, H. Blau, D. Melton, M. Moore, E.D. Thomas, C. Verfaillie, I. Weissman, M. West, eds., Elsevier Science, San Diego, CA, pp.703-709.
- Bernardos, R.L., Lentz, S.I., Wolfe, M.S., Raymond, P.A.. 2004. Notch-Delta signaling is required for spatial patterning and Müller glia differentiation in the zebrafish retina. Devel. Biol. (In press)
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