This laboratory works on four different developmental
problems. We are interested in the early development of neuronal
lineages from the embryonic neural crest; which appears transiently
during development and which is a source of peripheral nervous system
neurons, among many other cell types. We use specific monoclonal
antibodies and no-flow cytometry to isolate neural crest subpopulations.
We also study the role of the neurofibromatosis I gene (a tumor
suppressor gene) in neural crest development and neuronal/ melanocyte/Schwann
cell lineage specification and apoptosis, using mouse embryonic
stem cells. An additional line of research examines the role of
the embryonic hindbrain, periotic mesenchyme and neural crest in
shaping inner ear development and the roles of transcription factor
and growth factor genes (e.g. BMPs) and their antagonists, such
as Noggin, Chordin and DAN, in axis formation and development of
the embryonic otocyst. We examine these issues in four model systems:,
chick/quail; mouse/ immortalized inner ear cell lines from the Immortomouse
and the zebrafish.
Representative Publications:
- Gerlach-Bank, L.M., Cleveland, A.R. and Barald, K.F. (2004). DAN
Directs Endolymphatic Sac and Duct Outgrowth in the Avian
Inner Ear. DEVELOPMENTAL DYNAMICS 229:219-230.
- Barald, K.F. and Kelley, M.W. (2004). From Placode to Polarization:
New Tunes in Development of the Inner Ear. Development 131: 4119-4130.
- Germiller, J.A., Smiley, E. C., Ellis, A.D., Hoff, J.S., Deshmukh,
I., Allen, S.J. and Barald, K.F. (2004) Molecular characterization
of conditionally-immortalized cell lines derived from early mouse
embryonic inner ear. Developmental Dynamics In press.
- Thompson, D., Gerlach-Bank, L.M., Barald, K.F. and Koenig,
R.J. (2003). Retinoic
Acid Repression of BMP4 in Inner Ear Development. Molecular
and Cellular Biology. 23: 2277-2286.
- Warner, S.J., Hutson, M.H., Oh, S.-H., Gerlach-Bank, L.M.,
Lomax, M.I. and Barald, K.F (2003). Expression
of ZIC Genes in the Development of the Chick Inner Ear and Nervous
System. Developmental Dynamics 226: 702-711.
- Gerlach-Bank, L.M., Ellis, A.D., Noonen, B. and Barald, K.F.
(2002). Cloning
and expression analysis of the chick DAN gene, an antagonist
of the BMP family of growth factors. Dev. Dynam. 224: 109-115.
- Hutson MR, Lewis JE, Nguyen-Luu D, Lindberg KH, Barald KF: Expression
of Pax-2 and patterning of chick inner ear. J. Neurocytol.
1999 Oct;28(10/11):795-807.
- Gerlach LM, Hutson MR, Germiller JA, Nguyen-Luu D, Barald
KF: Addition
of the BMP4 antagonist, noggin disrupts avian inner ear development. Development
127: 45-54, 2000. (published on the web, Dec. 8, 1999)
- Fritzsch B, Barald KF and Lomax MI: Early embryology of
the vertebrate ear. In Rubel, E.W., Popper, A.N. and
Fay, R.R., eds Development of the Auditory System. Springer
Handbook
of Auditory Research, 1997. pp. 80-145.
- Barald KF, Lindberg KH, Hardiman K, Kavka AI, Lewis JE, Victor
JC, Gardner CA, Poniatowski A: Immortalized
cell lines from embryonic avian and murine otocysts: tools for
molecular studies of the developing inner ear. Int
J Dev Neurosci. 1997 Jul;15(4-5):523-40.
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