The Metabolomics Core Joins the Biomedical Research Core Facilities
The BRCF are very pleased to announce the latest addition to our core facilities, the Metabolomics Core. The Metabolomics Core measures concentrations of small molecules in biological samples on a recharge basis. We strive to provide GLP-quality analytical services at competitive prices, and regularly customize assays to fit our clients' needs.
Services include initial consultation, method development, data collection, interpretation, and presentation.
Welcome to the BRCF! To learn more about Metabolomics, visit their website.
|In Focus: |
Researching the Causes of Lupus
Professor of Medicine Bruce Richardson, M.D., Ph.D., is a Rheumatologist with a research interest in the mechanisms causing human systemic lupus erythematosus. Lupus is an autoimmune disease that primarily affects women, and is caused by agents in the environment that trigger the disease in genetically predisposed people. The environmental agents inhibit DNA methylation, a repressive epigenetic mechanism, in lymphocytes. DNA methylation silences genes that are inappropriate or detrimental to the function of any given cell, and inhibiting DNA methylation activates their expression. We found that inhibiting DNA methylation converts normal, antigen specific "helper" T lymphocytes into autoreactive, inflammatory, cytotoxic cells that cause the disease.
"The DNA Sequencing Core has been essential to the success of our studies in many ways," Professor Richardson explained. "Their state of the art technology allowed us to identify and map the DNA sequences that demethylate in T cells, confirming that their aberrant expression is due to DNA demethylation. We have done this using in vitro models, mouse models, and lymphocytes from women with lupus, all with the help of the DNA Sequencing Core."
Their most recent studies demonstrate that women are primarily affected by lupus because they have 2 X chromosomes, while men have an X and a Y, and the second X chromosome is normally silenced by DNA methylation. The second X demethylates in women lupus, causing overexpression of genes that contribute to the disease (men can't do this).
"These studies would have been impossible without the DNA core. The cost of the equipment and the expertise to run it is beyond what normal research grants (NIH, foundations, VA) can support," he said. "The equipment is also continually updated, permitting cutting edge work. I have found that when I write grants and need to explain to the reviewers how I will do the studies, all I need to say is that they will be done in the University of Michigan DNA Sequencing Core and provide them the link to their website. This always works. Finally, over the years I have been invited to take positions at other institutions. I have turned them down because they lack similar, institutionally supported core facilities."
Intergrating Flow Cytometry and Microscopy to Advance Discovery
Join the Flow Cytometry Core and Amnis Cytometry Systems on Tuesday, May 22 for an Imaging and Cytometry Seminar to explore technology and data from several example applications that enable functional studies in immunology, oncology, and stem cell research. These systems combine high-speed image capture with image quantification to create statistically powerful microscopy platforms, enabling robust discrimination of cells based on their appearance.
"Imaging + Cytometry" Seminar
Tuesday, May 22, 2012 from 1-2 p.m.
To learn more, download the event flyer.
|Save the dates: Upcoming Events
MICHR's Implementation Science Conference May 23-24, 2012
Registration is still open for the Michigan Institute for Clinical & Health Research's first annual conference on implementation science entitled Implementation Science: Moving Forward.
Held at the NCRC Building 18 Dining Hall, the conference is designed to foster discussions among various disciplines to address the science of implementation in healthcare.
See the full itinerary, location details, speakers, and registration information please visit the event website
Attend the ISN Symposium 2012: "Biology and the Kidney"
Join the International Society of Nephrology for its Forefront Symposia as the world's leading nephrologists and investigators from related medical areas come together to explore new scientific domains and the latest developments in kidney disease and biology.
June 7-10, 2012
Ann Arbor, Michigan
University of Michigan
Commercialization Symposium on June 1
What resources are available at the U-M and locally to license new medical breakthroughs? Besides launching a spin-off company, what other options are available to successfully take a therapy or device to market? These questions and more will be answered at the "Introduction to Life Science Technology Commercialization Symposium" for U-M faculty, postdocs, and grad students on Friday, June 1 at the North Campus Research Complex. Co-sponsored by BD and featuring a panel hosted by our own Connie Chang, attendance is free.
NEW DATE: Driving a Genomics Revolution Through Open Source Innovation
The BioArbor Meeting featuring Dr. Eric Schadt and Dr. Brian Athey has been rescheduled from May 9 to Wednesday, June 6. We have learned Dr. Schadt will be presented with the CINE Special Jury Award for his documentary, "The New Biology," at an award ceremony which also takes place on May 9.
Consequently, BioArbor has rescheduled the meeting to June 6 to accommodate this honor for Dr. Schadt. And as an added bonus we'll screen Dr. Schadt's documentary during the networking hour at BioArbor. We hope colleagues in the Ann Arbor area will be able to join us for this unique opportunity to share insights with two thought leaders in the increasingly high-profile world of genomics. Free. RSVP and Learn more.
In This Issue
|The Metabolomics Core Joins the BRCF! |
|Researching the Causes of Lupus |
|Attend a FREE Imaging & Cytometry Seminar |
|Save the dates! |
|Services Update |
|Services Update: DNA Sequencing|
The Pacific Biosciences RS.
The Biomedical Research Core Facilities recently invested in new equipment for the Microscopy & Image Analysis Laboratory, including:
- (2) Thermocyclers
- (2) Illumina Hi Seq 2000
- (2) Illumina cBot
- IntegenX Apollo 24 workstation
- (2) Eppendorf EP-Motion workstations
- Illumina iScan ("Beadstation")*
- Roche REMy System Attachment (for existing Biomek FX robot)*
- Nanodrop 2000 with Laptop PC, Optiplex 790 SFF, Vacufuge with Pump*
- Methylation Software for the Sequenom*
- Uninterruptable Power Supply*
- Additional Computers for Heavy Computational Processing of Next Gen DNA Sequencing Data
- Fluidigm Inc. DNA Preparation Device
- Ion Proton Personal Genome Machine with associated OneTouch sample prep system
- Pacific Biosciences 'RS'
- Qiagen PyroMark Q96 MD system
- ABI Model 3730XL Genetic Analyzer
- On Order: Ion Proton Sequencing System (Expected late 2012)
- On Order: QuantStudio System to perform high-throughput real-time PCR analyses
These equipment acquisitions dramatically extend the range of services we can provide. We now have sequencers that fit most of the niches previously uncovered. We have dramatically increased our equipment for assessment of DNA methylation. Finally, we have expanded our ability to prepare samples for the previously-existing sequencing systems. The net effect is that the U-M DNA Sequencing Core is now much more of a full-service facility." - Bob Lyons, Ph.D., Director, DNA Sequencing Core