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Quick Facts
Transgenic Animal Model
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| Service | Rate (U-M Units) | Notes |
| B6SJLF2 Hybrid Transgenic Mouse Production | $3,000 | Three transgenic mice are guaranteed. Mice are produced in the (C57BL/6 X SJL)F2 genetic background. Other genetic backgrounds may be specified by investigators (additional cost). Large DNAs (BACs, YACs, P1s) may be specified at no additional cost. |
| C57BL/6J Transgenic Mouse Production | $5,400 | Three transgenic mice are guaranteed. Mice are produced in the (C57BL/6 X SJL)F2 genetic background. Other genetic backgrounds may be specified by investigators (additional cost). Large DNAs (BACs, YACs, P1s) may be specified at no additional cost. |
| SD Outbred Transgenic Rat Production | $7,500 | Three transgenic rats are
guaranteed. Rats are produced in the Sprague-Dawley genetic background (Crl:CD (SD)IGS BR ). Other genetic backgrounds may be specified by investigators (additional cost). Large DNAs (BACs, YACs, P1s) may be specified at no additional cost. |
| F344/NHsd Transgenic Rat Production | $10,100 | Three transgenic rats are
guaranteed. Rats are produced in the Sprague-Dawley genetic background (Crl:CD (SD)IGS BR ). Other genetic backgrounds may be specified by investigators (additional cost). Large DNAs (BACs, YACs, P1s) may be specified at no additional cost. |
| ES Cell Electroporation | $4,100 | |
| Transient Cre or FLP ES Cell Electroporation | $820 | |
| ES Clone Expansion | $530 per clone | |
| KOMP or Genetrap ES Clone Expansion | $530 | |
| Blastocyst Microinjection of C57BL/6 ES Cells | $2,000 | |
| Blastocyst Microinjection of 129 Strain ES Cells | $1,900 | |
| De Novo ES Cell Line Derivation | $2,100 | Preparation of ES cell lines from mouse blastocysts. Preparation of ES cell lines from mouse blastocysts. Preparation of ES cell lines from mouse blastocysts.We will collect blastocysts from your genetically engineered mouse model and culture them under conditions that are conducive to establishing ES cell lines. The number of ES cell lines produced is propotional to the number blastoycsts available for culture. The success of the Transgenic Core in de novo ES cell line derivation meets or exceeds published standards. |
| Rederivation of Pathogen Free Mouse Lines | $810 | |
| Mouse Speed Cryo | $900 | |
| Mouse 8-Cell Egg Cryopreservation | $900 per session | |
| Mouse Strain Cryo-recovery | $790 | |
| Mouse Sperm Cryo | $620 | |
| BAC Recombineering | $2,000 | Acquistion of BACs from BAC
library resources. Purification of BAC DNA suitable for pronuclear
microinjection (transgenic mice or transgenic rats). Pulsed field gel mapping
of BAC DNA to confirm identiy of BAC. Genetic modification of BACs by recombineering approaches to produce
gene knockins, knockouts. Prepareation of gene targeting vectors for the
modifcation of genes in embyronic stem cells. Discuss structure of the desired BAC with the investigator and propose a recombineering path. Plasmids for knockins of fluorescent reporter molecules and Cre recombinase are available. |
