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Peptide and Proteomics

The goal of the Peptide and Proteomics Core is to enhance and stimulate research in diabetes, its complications and related endocrine and metabolic disorders by MDRTC investigators by providing the broadest range of resources for the analysis of peptides and proteins. This core provides assistance with peptide synthesis, including multiply labeled, cyclic peptides and peptides with posttranslational modifications. N-terminal sequence analysis is offered for protein characterization as is Circular Dichroism Spectroscopy and state-of-the-art technology in protein analysis such as routine identification of proteins by mass spectrometry. Proteomic services include proteome mapping, protein expression profiling, protein complexes, and post-translational modifications. Technologies offered include 1) protein expression profiling using isotope enrichment, ion statistics, and two dimensional gel electrophoresis; 2) phosphorylation site mapping as well as mapping of other post-translational modifications; 3) MuDPIT analyses; 4) 1D and 2D capillary HPLC; 5) bioinformatics support and training; 6) tandem mass spectrometry; and 7) proteome informatics. The Core maintains an information management system for proteomics, providing an environment for investigators to access and curate their data.

Proteomic Services

The MDRTC recently contracted with the Proteomics Facility Core at Wayne State University to provide state-of-the-art technology for characterization of protein
identity, modification and differential expression and to serve as a resource for training, education and consultation for proteomic applications.  The Proteomics Core, under the direction of Paul M. Stemmer, PhD, Associate Professor, Wayne State University, will provide the following services for MDRTC members:

Investigators planning large projects are encouraged to speak with either Dr. Stemmer, core director, (313-577-6536) or Dr. Joseph Caruso, co-director, (313-577-6542) before submitting samples.  

For general inquiries about sample submission, please contact the laboratory at 313-577-6545.

A submission form should be included with all samples. That form is available on the Proteomics Facility Core web site at http://proteomics.wayne.edu/submissions.php
 
Studies involving human subjects must submit documentation of IRB approval with the samples.

Studies that require IACUC approval must include the approved investigator’s name and the approval number with the samples. 

Samples should be mailed with ice packs or dry ice to:

Wayne State University
Proteomics Facility Core
Room 2105, Scott Hall of Medical Sciences
540 East Canfield
Detroit, MI, USA, 48201

Samples are accepted by the core Monday through Friday.  Morning delivery should be selected for samples that need to be opened on the day of delivery. 

The fee structure for these services may be found at http://proteomics.wayne.edu/services-fees.php.  The MDRTC will provide discounts for Proteomics Facility Core services for diabetes-related projects. Initially, a discount of 50% with a cap of $3000/investigator will be given.  Discounts will be reassessed as we gain more experience with usage.  To have your project reviewed for diabetes-relatedness, please e-mail Martin G. Myers, MD, PhD at mgmyers@umich.edu/. 

For complete information on the equipment, analysis tools, services and
fees, submission criteria, etc. please go to http://proteomics.wayne.edu/.

Recharge Rates

Charges for U-M-MDRTC investigators for all services are reduced by 75%. For a list of recharge rates for Protein Mass Spectrometry, click here. For a list of recharge rates for Peptide Synthesis, click here.

Services

A. Protein Mass Spectrometry and Analysis:

B. Peptide Synthesis (available at the Protein Structure Facility)

Equipment:

Sample Preparation, Request Submission and Other Information:

Key Personnel

Henriette Remmer, Ph.D., Co-director, Peptide and Proteomics Core,
hremmer@umich.edu; 734-763-6285